Microchip-Based Single-Cell Imaging Reveals That CD56dimCD57-KIR-NKG2A+ NK Cells Have More Dynamic Migration Associated with Increased Target Cell Conjugation and Probability of Killing Compared to CD56dimCD57-KIR-NKG2A- NK Cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
Elin ForslundBjörn Önfelt

Abstract

NK cells are functionally educated by self-MHC specific receptors, including the inhibitory killer cell Ig-like receptors (KIRs) and the lectin-like CD94/NKG2A heterodimer. Little is known about how NK cell education influences qualitative aspects of cytotoxicity such as migration behavior and efficacy of activation and killing at the single-cell level. In this study, we have compared the behavior of FACS-sorted CD56(dim)CD57(-)KIR(-)NKG2A(+) (NKG2A(+)) and CD56(dim)CD57(-)KIR(-)NKG2A(-) (lacking inhibitory receptors; IR(-)) human NK cells by quantifying migration, cytotoxicity, and contact dynamics using microchip-based live cell imaging. NKG2A(+) NK cells displayed a more dynamic migration behavior and made more contacts with target cells than IR(-) NK cells. NKG2A(+) NK cells also more frequently killed the target cells once a conjugate had been formed. NK cells with serial killing capacity were primarily found among NKG2A(+) NK cells. Conjugates involving IR(-) NK cells were generally more short-lived and IR(-) NK cells did not become activated to the same extent as NKG2A(+) NK cells when in contact with target cells, as evident by their reduced spreading response. In contrast, NKG2A(+) and IR(-) NK cells showed similar dyn...Continue Reading

References

Jan 1, 1989·Advances in Immunology·G Trinchieri
Jul 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joseph D MillerPeter E Jensen
Aug 12, 2006·Immunity·Nicolas AnfossiEric Vivier
Oct 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Junli YuKatharine C Hsu
Oct 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cyril FauriatKarl-Johan Malmberg
Jan 28, 2009·The Journal of Experimental Medicine·Timothy J NiceDavid H Raulet
Jul 29, 2009·PLoS Biology·Fiona J CulleyDaniel M Davis
Aug 25, 2009·European Journal of Immunology·Kerstin JuelkeChiara Romagnani
Jun 21, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Mohammad Ali KhorshidiBjörn Önfelt
Feb 26, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Athanasia E ChristakouBjörn Önfelt
Sep 17, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·L Michael ThomasEric O Long
Jan 22, 2014·Immunology and Cell Biology·Emily M MaceJordan S Orange
Mar 19, 2014·Frontiers in Immunology·Per E OlofssonBjörn Onfelt
Apr 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Monika EnqvistKarl-Johan Malmberg

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Citations

Mar 15, 2016·Frontiers in Immunology·Dara M Strauss-Albee, Catherine A Blish
Apr 20, 2016·Frontiers in Immunology·Karolin GuldevallBjörn Önfelt
Jun 7, 2016·Cellular & Molecular Immunology·Yuke He, Zhigang Tian
Oct 13, 2017·Molecular Biology of the Cell·Barclay J Lee, Emily M Mace
Oct 27, 2018·Seminars in Immunopathology·Michelle L SaetersmoenKarl-Johan Malmberg
Jul 25, 2019·Scientific Reports·Per E OlofssonBjörn Önfelt
Nov 27, 2016·Cancer Immunology, Immunotherapy : CII·Ji Sung KimSang-Bae Han
Oct 19, 2017·Scientific Reports·Emelie FogelqvistHans M Hertz
Feb 2, 2019·Nature Communications·Jodie P GoodridgeKarl-Johan Malmberg
May 13, 2020·Immune Network·Min Hwa ShinKyung-Mi Lee
Mar 29, 2020·Immunogenetics·Frederick J Goodson-GreggStephen K Anderson
Jul 28, 2020·Cancers·Dhon Roméo MakangaChristelle Retière
Dec 28, 2017·Bioconjugate Chemistry·HyoungJun ParkJunsang Doh

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