Microchip micellar electrokinetic chromatography separation of alkaloids with UV-absorbance spectral detection

Electrophoresis
Carl I D NewmanGreg E Collins

Abstract

A microchip device is demonstrated for the electrophoretic separation and UV-absorbance spectral detection of four toxic alkaloids: colchicine, aconitine, strychnine, and nicotine. A fused-silica (quartz) microchip containing a simple cross geometry is utilized to perform the separations, and a miniature, fiber-optic CCD spectrometer is coupled to the microchip for detection. Sensitive UV-absorbance detection is achieved via the application of online preconcentration techniques in combination with the quartz microchip substrate which contains an etched bubble-cell for increased pathlength. The miniature CCD spectrometer is configured to detect light between 190 and 645 nm and LabView programming written in-house enables absorbance spectra as well as separations to be monitored from 210 to 400 nm. Consequently, the configuration of this microchip device facilitates qualitative and quantitative separations via simultaneous spatial and spectral resolution of solutes. UV-absorbance limits of quantification for colchicine, 20 microM (8 mg/L); strychnine, 50 microM (17 mg/L); aconitine, 50 microM (32 mg/L); and nicotine, 100 microM (16 mg/L) are demonstrated on the microchip. With the exception of aconitine, these concentrations are ...Continue Reading

References

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Apr 15, 2007·Talanta·Greg E CollinsPeter Wu

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Citations

Feb 16, 2013·Chemical Reviews·Pamela N NgeAdam T Woolley
Oct 31, 2009·Journal of Separation Science·María Asensio-RamosSalvatore Fanali
Oct 18, 2008·Journal of Separation Science·Christine L CopperGreg E Collins
Jan 9, 2009·Electrophoresis·Alberto EscarpaMiguel García
Dec 23, 2008·Electrophoresis·Zdena MaláPetr Bocek
Dec 8, 2011·Electrophoresis·Heide R RabanesJoselito P Quirino
Jul 3, 2013·Biomedical Chromatography : BMC·Szymon DziombaTomasz Bączek
Mar 7, 2013·Electrophoresis·Entesar Al-Hetlani
Feb 7, 2012·Analytica Chimica Acta·Braden C GiordanoAlex Terray
Oct 8, 2008·Analytical Chemistry·Joseph F BorowskyGreg E Collins

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