Microcin J25 uptake: His5 of the MccJ25 lariat ring is involved in interaction with the inner membrane MccJ25 transporter protein SbmA.

Journal of Bacteriology
Ricardo E de CristóbalRicardo N Farías

Abstract

Escherichia coli microcin J25 (MccJ25) is a plasmid-encoded antibiotic peptide consisting of 21 L-amino acid residues (G1-G-A-G-H5-V-P-E-Y-F10-V-G-I-G-T15-P-I-S-F-Y20-G). E. coli RNA polymerase (RNAP) is the intracellular target of MccJ25. MccJ25 enters cells after binding to specific membrane transporters: FhuA in the outer membrane and SbmA in the inner membrane. Here, we studied MccJ25 mutants carrying a substitution of His5 by Lys, Arg, or Ala. The inhibitory effects on cellular growth and in vitro RNAP activity were determined for each mutant microcin. The results show that all mutants inhibited RNAP in vitro. However, the mutants were defective in their ability to inhibit cellular growth. Experiments in which the FhuA protein was bypassed showed that substitutions of MccJ25 His5 affected the SbmA-dependent transport. Our results thus suggest that MccJ25 His5 located in the lariat ring is involved, directly or indirectly, in specific interaction with SbmA and is not required for MccJ25 inhibition of RNAP.

References

Nov 1, 1992·Journal of Bacteriology·R A Salomón, R N Farías
Jun 1, 1995·Journal of Bacteriology·R A Salomón, R N Farías
Dec 1, 1993·Journal of Bacteriology·R A Salomón, R N Farías
Jun 1, 1996·Journal of Bacteriology·J O SolbiatiR A Salomón
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·N OpalkaS A Darst
Jul 10, 2001·Journal of Bacteriology·M A DelgadoR A Salomón
Oct 29, 2002·The Journal of Biological Chemistry·Julia YuzenkovaKonstantin Severinov
Nov 13, 2002·Microbiology·Helmut KillmannVolkmar Braun
Mar 28, 2003·Biochemical and Biophysical Research Communications·Augusto BellomioRoberto D Morero
Oct 9, 2003·Journal of the American Chemical Society·Marvin J BayroRichard H Ebright
Oct 9, 2003·Journal of the American Chemical Society·K Johan RosengrenDavid J Craik
Oct 9, 2003·Journal of the American Chemical Society·Kelly-Anne WilsonSeth A Darst
Jun 18, 2004·Molecular Cell·Karen AdelmanKonstantin Severinov
Nov 24, 2004·Biochemical and Biophysical Research Communications·Augusto BellomioRicardo N Farías
Apr 27, 2005·Biochemical and Biophysical Research Communications·Paula A VincentRoberto D Morero

❮ Previous
Next ❯

Citations

Jul 27, 2007·Natural Product Reports·Sophie DuquesneSylvie Rebuffat
Nov 26, 2010·Protein Engineering, Design & Selection : PEDS·Si Jia PanA James Link
Aug 31, 2010·Applied and Environmental Microbiology·María Fernanda PomaresPaula A Vincent
Sep 11, 2007·Journal of Molecular Microbiology and Biotechnology·Sophie DuquesneSylvie Rebuffat
Aug 30, 2007·Molecular Microbiology·Maura MattiuzzoMarco Scocchi
Aug 23, 2007·Molecular Microbiology·Konstantin SeverinovMikhail S Gelfand
Nov 28, 2012·Biochemical Society Transactions·Indran Mathavan, Konstantinos Beis
Dec 4, 2014·Amino Acids·Riadh HammamiIsmail Fliss
Sep 18, 2009·FEMS Microbiology Letters·Miriam C ChalonPaula A Vincent
Jan 13, 2010·Chemistry & Biology·Thomas A KnappeMohamed A Marahiel
Jan 2, 2010·FEBS Letters·Thomas A KnappeMohamed A Marahiel
Jun 18, 2017·Journal of Chemical Theory and Computation·Pin-Kuang Lai, Yiannis N Kaznessis
Jan 31, 2012·Chembiochem : a European Journal of Chemical Biology·Rémi DucasseSéverine Zirah
Mar 1, 2007·Genetika·O A Pavlova, K V Severinov
May 26, 2017·Natural Product Reports·Michael GrafDaniel N Wilson
Dec 17, 2020·Chemical Biology & Drug Design·Maryam Mazaheri TehraniNour Amirmozafari
May 1, 2021·Journal of Industrial Microbiology & Biotechnology·Li CaoA James Link
May 15, 2018·ACS Chemical Biology·Maria RomanoKonstantinos Beis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Organic & Biomolecular Chemistry
David J Clarke, Dominic J Campopiano
AJR. American Journal of Roentgenology
Constance D LehmanJennifer Shook
Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
M E LaddG C McKinnon
© 2022 Meta ULC. All rights reserved