PMID: 2489088Aug 1, 1989Paper

Micrococcal nuclease digestion study of spermidine-condensed DNA

International Journal of Biological Macromolecules
K A Marx, T C Reynolds

Abstract

Spermidine-condensed lambda DNA tertiary structures have been studied by micrococcal nuclease digestion. Broad but discrete DNA bands were observed in gel electrophoresis experiments of digests at sizes of: 1003 +/- 115 bp, 1972 +/- 190 bp and 3100 +/- 350 bp. These bands comprise an arithmetic series, similar to, but larger than, arithmetic DNA band series sizes we have observed previously in calf thymus and phi x-174 DNA condensates. The 1003bp monomer lambda DNA band size corresponds to wrapping B DNA once circumferentially about the toroidal-shaped tertiary structures, the predominant condensed structures present in these preparations, and is consistent with the measured electron microscopic dimensions for hydrated lambda DNA toruses previously presented. DNA fragment length stability was determined by release from the digested condensates. Fragments of 80-85bp and sizes below are thermodynamically unstable in the lambda DNA condensates. This fragment size agrees well with a recent determination of the cooperativity size in DNA condensates.

References

May 29, 1979·Biochemistry·R W Wilson, V A Bloomfield
Sep 23, 1977·Biochemical and Biophysical Research Communications·K A Marx
May 25, 1978·Journal of Molecular Biology·D K ChattorajA Schellman
Aug 1, 1985·Biopolymers·G A GriessP M Horowitz
Aug 5, 1973·Journal of Molecular Biology·K E RichardsR Calendar
Apr 29, 1980·Biochemistry·L C Yip, M E Balis
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K A Marx, T C Reynolds
Oct 9, 1984·Biochemistry·D Porschke
Dec 25, 1983·Journal of Molecular Biology·J Widom, R L Baldwin
Dec 25, 1980·Journal of Molecular Biology·J Widom, R L Baldwin
Apr 1, 1983·Biopolymers·T J Thomas, V A Bloomfield
Nov 1, 1982·Biopolymers·C B Post, B H Zimm
Jun 25, 1981·Nucleic Acids Research·W Hörz, W Altenburger

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