Micrococcus luteus, a bacterium with a high genomic G + C content, contains Escherichia coli-type promoters.

Molecular & General Genetics : MGG
M NakayamaA Ishihama

Abstract

The G + C content of Micrococcus luteus DNA is 74%, which is much higher than that of Escherichia coli (about 50%). In order to understand the influence of GC-directed mutation pressure (GC pressure) on the promoter structure, the initiation sites for both in vitro and in vivo transcription of M. luteus streptomycin (str) and spectinomycin (spc) operons were identified by the reverse transcriptase mapping method. The promoter sequences of M. luteus are similar to those of E. coli, but have significantly higher G + C contents. In an in vitro run-off transcription assay using truncated DNA templates, RNA polymerases from both M. luteus and E. coli were able to transcribe correctly from both the str promoter and the spc major promoter of M. luteus.

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A Muto, S Osawa
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
Apr 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·N SUEOKA

❮ Previous
Next ❯

Citations

Jan 1, 1993·FEMS Microbiology Reviews·P Steinrücke, B Ludwig
Dec 12, 2003·Computational Biology and Chemistry·Rupali N KalateBhaskar D Kulkarni
Jun 1, 1996·Journal of Bacteriology·T J Kenney, G Churchward
Jul 1, 1991·Journal of Bacteriology·B J MacGregor, T J Donohue
Jan 1, 1997·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·M A MulderL M Steyn
Nov 9, 2005·Bioorganic & Medicinal Chemistry Letters·Humberto González-DíazYenny González-Díaz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.