PMID: 6257274Oct 28, 1980Paper

Micrococcus luteus endonucleases for apurinic/apyrimidinic sites in deoxyribonucleic acid. 2. Further studies on the substrate specificity and mechanism of action

J Pierre, J Laval


Two endonucleases specific for DNA-containing apurinic or apyrimidinic sites (AP-endonucleases A and B) have been isolated from Micrococcus luteus and highly purified. These enzymes have no exonuclease activity. Both AP-endonucleases hydrolyze DNA-containing apurinic or apyrimidinic sites at the 5' end of the lesion, thus generating 3'-hydroxyl and 5'-phosphoryl end groups. DNA-containing pyrimidine dimers, introduced at low doses of UV, are not hydrolyzed, whereas DNA-containing lesions, introduced at high doses of UV or by gamma irradiation are nicked by either AP-endonuclease. During hydrolysis of apurinic DNA, neither of the AP-endonucleases acts as a processive enzyme.


Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J LavalF Laval
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·N J DukerD E Fishbein
Nov 15, 1996·Nucleic Acids Research·D Proudnikov, A Mirzabekov
Aug 1, 1982·Biochimie·L Grossman, R H Grafstrom
Apr 1, 1986·Experimental and Molecular Pathology·N J Duker, P E Gallagher
Jan 1, 1988·Environmental and Molecular Mutagenesis·S S Wallace

Related Concepts

Alkaline DNase
DNA, Viral
X-Rays, Nuclear
Substrate Specificity
Black Light, Ultraviolet
Deoxyribonuclease IV (Phage T4-induced)
DNA-(apurinic or apyrimidinic site) lyase

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