Microcystin-LR Alters the Gene Transcription and Activities of Iodothyronine Deiodinases in the Hepatic Cells of Grass Carp (Ctenopharyngodon Idella)

Journal of Biochemical and Molecular Toxicology
Zidong LiuDapeng Li

Abstract

This study investigated the gene transcription and activities of iodothyronine deiodinases in the hepatic cell line of grass carp (Ctenopharyngodon idella) exposed in vitro to 1, 10, 100, and 1000 μg /L microcystin-LR (MC-LR) for either 24 or 48 h. The cell viabilities were not significantly affected by MC-LR exposure. The mRNA expressions of type I iodothyronine deiodinase (ID1) and type Ⅱ iodothyronine deiodinase (ID2) reduced after the exposure to MC-LR. However, MC-LR exposure led to the increase in the mRNA expression of type Ⅲ iodothyronine deiodinase (ID3). Moreover, significant ID1 and ID2 activities decline were also observed in the hepatic cell line of grass carp exposed to MC-LR, and the activity of ID3 increased significantly in the MC-LR treated groups. The results suggested that MC-LR could alter the gene transcription or activities of IDs in the hepatic cell line of grass carp.

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Citations

Nov 2, 2015·Journal of Hazardous Materials·Liang ChenPing Xie

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