Microcystin-LR Does Not Alter Cell Survival and Intracellular Signaling in Human Bronchial Epithelial Cells.

Toxins
Ondřej BrózmanPetra Laboha

Abstract

Changes in ecological and environmental factors lead to an increased occurrence of cyanobacterial water blooms, while secondary metabolites-producing cyanobacteria pose a threat to both environmental and human health. Apart from oral and dermal exposure, humans may be exposed via inhalation and/or swallowing of contaminated water and aerosols. Although many studies deal with liver toxicity, less information about the effects in the respiratory system is available. We investigated the effects of a prevalent cyanotoxin, microcystin-LR (MC-LR), using respiratory system-relevant human bronchial epithelial (HBE) cells. The expression of specific organic-anion-transporting polypeptides was evaluated, and the western blot analysis revealed the formation and accumulation of MC-LR protein adducts in exposed cells. However, MC-LR up to 20 μM neither caused significant cytotoxic effects according to multiple viability endpoints after 48-h exposure, nor reduced impedance (cell layer integrity) over 96 h. Time-dependent increase of putative MC-LR adducts with protein phosphatases was not associated with activation of mitogen-activated protein kinases ERK1/2 and p38 during 48-h exposure in HBE cells. Future studies addressing human health ri...Continue Reading

References

Jan 1, 1992·Journal of Cancer Research and Clinical Oncology·R Nishiwaki-MatsushimaH Fujiki
Jan 1, 1994·American Journal of Respiratory Cell and Molecular Biology·A L CozensD C Gruenert
May 11, 1993·The American Journal of Physiology·J R YankaskasR C Boucher
Feb 21, 1998·Australian and New Zealand Journal of Public Health·L S PilottoR G Attewell
May 20, 2000·Chemosphere·M W ChongW F Fong
Jun 30, 2000·Biochemical and Biophysical Research Communications·I TamaiA Tsuji
Nov 28, 2002·Chemico-biological Interactions·Andrey MikhailovJohn E Eriksson
Dec 7, 2002·Toxicon : Official Journal of the International Society on Toxinology·Bojana ZeguraMetka Filipic
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·D C GruenertR A Frizzell
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Pei-Jen WangShyh-Jye Lee
Mar 2, 2005·Toxicology and Applied Pharmacology·W J FischerB Hagenbuch
Mar 2, 2005·Toxicology and Applied Pharmacology·Geoffrey A CoddJames S Metcalf
Apr 5, 2005·Toxicon : Official Journal of the International Society on Toxinology·Janet M BensonLora E Fleming
Aug 12, 2006·The Lancet Oncology·Yann GrosseUNKNOWN WHO International Agency for Research on Cancer Monograph Working Group
Nov 23, 2006·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·K BleasbyJ G Slatter
May 25, 2007·Toxicon : Official Journal of the International Society on Toxinology·Raquel M SoaresSandra M F O Azevedo
Jul 5, 2008·Nature Protocols·Guillermo RepettoJorge L Zurita
Jul 21, 2009·Toxicon : Official Journal of the International Society on Toxinology·Lorraine C BackerYung-Sung Cheng
Jun 16, 2010·Toxicon : Official Journal of the International Society on Toxinology·Giovanna M C CarvalhoWalter A Zin
Apr 28, 2012·Journal of Applied Toxicology : JAT·Xin ZhangYan Cai
Jun 8, 2012·Annals of the New York Academy of Sciences·Christian E OvergaardMichael Koval
Sep 27, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Carina MenezesPaulo Pereira
Jan 15, 2013·Microbial Ecology·Hans W Paerl, Timothy G Otten
Mar 19, 2013·Oncogene·J StebbingG Giamas
Jan 4, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Mateja Erdani KreftKatja Kristan
May 20, 2015·Toxicon : Official Journal of the International Society on Toxinology·Mi ZhouJin Xu
Jun 20, 2015·Toxins·Zhu ZhuGuoqing Shi
Jun 30, 2015·Current Protein & Peptide Science·Feng QianJohn W Christman
Jul 30, 2015·Toxicon : Official Journal of the International Society on Toxinology·Vinícius R OliveiraWalter A Zin
Sep 19, 2015·Environmental Science & Technology·Ondrej AdamovskyLudek Blaha
Nov 2, 2015·Journal of Hazardous Materials·Liang ChenPing Xie
Feb 5, 2016·Toxicon : Official Journal of the International Society on Toxinology·Giovanna Marcella Cavalcante CarvalhoWalter Araujo Zin
Mar 1, 2016·Journal of Toxicology·Yaqoob LoneRaj Kumar Koiri
Mar 10, 2016·Mini Reviews in Medicinal Chemistry·Bojana Zegura
Mar 21, 2016·Toxicon : Official Journal of the International Society on Toxinology·Cong WangXiaodong Han
Apr 3, 2016·Environmental Pollution·Qin WuGuangyu Li
Jun 30, 2016·Nature Reviews. Molecular Cell Biology·Ceniz ZihniMaria S Balda
Jul 26, 2016·Experimental and Therapeutic Medicine·Yang LiXiaoli Fu

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Citations

Dec 19, 2020·Environmental Science & Technology·Haley E Plaas, Hans W Paerl

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Methods Mentioned

BETA
PCR
transfection
electrophoresis
Protein Assay

Software Mentioned

MS Excel
xCelligence
ImageJ
GelCapture
SigmaPlot

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