Microcystin-LR induces toxic effects in differentiated and undifferentiated Caco-2 cells

Archives of Toxicology
María PuertoAna M Cameán

Abstract

Microcystins (MCs) are toxins of heptapeptidic structure produced by toxic cyanobacteria in surface eutrophic waters. MCs are known to be hepatotoxic in humans, but they are also able to induce gastrointestinal alterations, allergic reactions, irritation, and pneumonia-like symptoms. The impact of MC-LR, one of the most common cyanobacterial toxins, was studied on the Caco-2 cell line, a commonly used enterocytic model, established from a human colon carcinoma. Caco-2 cells were differentiated in order to compare the effect of MC-LR in differentiated and non-differentiated cells. They were seeded in a 96-well microtiter plate and treated with MC-LR pure standard (98% purity). The effects of different concentrations of this cyanotoxin (50, 100, 150, and 200 microM) were investigated at 24 and 48 h of exposure by morphological observation and biochemical changes (total protein content, neutral red uptake, and MTS metabolization). Differentiated Caco-2 cells were slightly more sensitive than undifferentiated cells. Moreover, toxic effects induced by MCs were higher at 48 h compared to those observed at 24 h. The most sensitive endpoint for the cell line was the reduction of total protein content. Morphological changes induced by M...Continue Reading

References

Jun 1, 1992·The Journal of Applied Bacteriology·W W Carmichael
Jan 1, 1990·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P Boyle
Dec 31, 1990·Biochemical and Biophysical Research Communications·J E ErikssonD Hartshorne
Sep 1, 1990·Pharmaceutical Research·A R HilgersP S Burton
Aug 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·K E FladmarkS O Døskeland
May 20, 2000·Chemosphere·M W ChongW F Fong
Aug 23, 2003·Aquatic Toxicology·Tina BatistaDusan Suput
Oct 16, 2003·Toxicon : Official Journal of the International Society on Toxinology·V F MagalhãesS M F O Azevedo
Jan 7, 2004·Mutation Research·Li ZhanMasamitsu Honma
Mar 24, 2004·Toxicon : Official Journal of the International Society on Toxinology·Nicolette BothaEnid G Shephard
Mar 2, 2005·Toxicology and Applied Pharmacology·Stefan J HoegerDaniel R Dietrich
Mar 2, 2005·Toxicology and Applied Pharmacology·W J FischerB Hagenbuch
Apr 5, 2005·Toxicon : Official Journal of the International Society on Toxinology·Ivanka TenevaKristin Schirmer
Aug 6, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S PichardoG Repetto
May 23, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yoshimichi SaiAkira Tsuji
Aug 21, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S PichardoG Repetto

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Citations

Jul 24, 2012·Chemosphere·Daniel Gutiérrez-PraenaAna María Cameán
Dec 4, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Daniel Gutiérrez-PraenaAna M Cameán
Dec 3, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Adeline TarantiniValérie Fessard
Sep 5, 2019·Toxins·Linghui CaoFei Yang

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