Microdialysis for evaluating the entrapment and release of a lipophilic drug from nanoparticles

Journal of Pharmaceutical and Biomedical Analysis
C B MichalowskiTeresa Dalla Costa

Abstract

The aim of the study was to evaluate the microdialysis (MD) as a tool to determine entrapment efficiency and drug release of a lipophilic drug model, diclofenac (DIC), from nanocapsules, nanospheres, and nanoemulsions. Factors that could interfere with the MD probe recovery were investigated: perfusion fluid composition, concentration and form of the drug in the perfusate, and recovery method. DIC entrapment efficiency to nanoparticles and the drug release in phosphate buffer pH 7.4 after different dilutions were evaluated by MD and ultrafiltration/centrifugation (UC). DIC recovery for the 5 microL/min flux was concentration and pH dependent. DIC sodium was used for the recoveries determination since it did not differ from the DIC acid recovery for the same media. DIC entrapment efficiency determined applying both techniques were equivalent and close to 100% for all nanoparticles. In pH 7.4 DIC release from the nanoparticles was partial for the dilution rate 1:1 (v/v), around 50-60%. A complete release was observed from 1:10 (v/v) dilution. Only nanocapsules presented a incomplete release for 1:5 (v/v) dilution, around 86%. MD and UC techniques were equivalent for the evaluation of DIC entrapment efficiency and drug release fro...Continue Reading

Citations

Sep 30, 2014·International Journal of Environmental Research and Public Health·Evelyn WinterTânia B Creckzynski-Pasa
Dec 1, 2007·Journal of Biological Physics·Carolina Morales MoraesLeonardo Fernandes Fraceto
May 30, 2014·AAPS PharmSciTech·Fatemeh BahadoriHayat Onyüksel
Sep 27, 2013·Drug Delivery and Translational Research·Jie Shen, Diane J Burgess
Jul 9, 2008·Journal of Microencapsulation·Xiaoliang LiuHongyao Zhang
Apr 15, 2009·Drug Development and Industrial Pharmacy·Fang LiXing Tang
Jul 6, 2010·Current Eye Research·Eliana B SoutoMaria Luisa Garcia
Feb 22, 2008·International Journal of Pharmaceutics·Jie LiWeisan Pan
Jun 22, 2007·International Journal of Pharmaceutics·Regina G KelmannLetícia S Koester
Feb 13, 2007·International Journal of Pharmaceutics·Sávia Caldeira de AraújoMônica Cristina de Oliveira
Jan 25, 2020·The Journal of Pharmacy and Pharmacology·Ayse Karatug KacarSehnaz Bolkent
Nov 3, 2020·Frontiers in Molecular Biosciences·Jay V ShahVidya Ganapathy
Oct 5, 2020·Journal of Pharmaceutical and Biomedical Analysis·Yuchen FanKelly Zhang
Dec 30, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shu-Pei HuangTung-Hu Tsai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.