PMID: 3319884Oct 1, 1987Paper

Microdissection and microcloning from the proximal region of mouse chromosome 7: isolation of clones genetically linked to the pudgy locus

Genomics
A J Greenfield, S D Brown

Abstract

Microdissection and microcloning have been utilized in order to create a bank of clones from the proximal region of mouse chromosome 7. Several important loci map to this area, including the albino locus (c), pink-eye dilution (p), and the developmental mutant, pudgy (pu). By use of interspecific crosses between Mus musculus domesticus and Mus spretus, we have generated backcross progeny segregating for the mutations chinchilla (cch) and pink-eye dilution (p). Exploiting the evolutionary divergence between the two species, we have analyzed the inheritance of restriction fragment length variants of three microclones and their linkage to the two markers cch and p, respectively. All three clones studied map to the dissected region, and as such also show genetic linkage to the pudgy locus. This bank of chromosome 7-derived microclones should provide molecular start points for the isolation of a variety of developmental loci of unknown gene product, including the pudgy locus.

References

Nov 1, 1986·Molecular and Cellular Biology·G P BatesS D Brown
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E M FisherS D Brown
Jan 1, 1974·Cold Spring Harbor Symposia on Quantitative Biology·M BotchanP A Sharp
Aug 1, 1965·Developmental Biology·R L SidmanC Waymouth

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Citations

May 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R D Saunders
Feb 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F T Kao
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·E M RinchikJ Peters
Jan 1, 1991·Mammalian Genome : Official Journal of the International Mammalian Genome Society·E M RinchikJ Peters
Mar 1, 1990·Somatic Cell and Molecular Genetics·J WeberK Olek
Jan 1, 1991·Annals of the New York Academy of Sciences·S D BrownK P Steel
May 10, 2008·Genome Génome / Conseil National De Recherches Canada·R ZimmerA M Gibbins
Jan 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·T S SonstegardC W Beattie

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