Microdissection of the Ah01 chromosome in upland cotton and microcloning of resistance gene anologs from the single chromosome

Hereditas
Xinchuan CaoKunbo Wang

Abstract

Chromosome microdissection is one of the most important techniques in molecular cytogenetic research. Cotton (Gossypium Linnaeus, 1753) is the main natural fiber crop in the world. The resistance gene analog (RGA) cloning after its single chromosome microdissection can greatly promote cotton genome research and breeding. Using the linker adaptor PCR (LA-PCR) with the primers of rice disease-resistance homologues, three nucleotide sequences PS016 (KU051681), PS054 (KU051682), and PS157 (KU051680) were obtained from the chromosome Ah01 of upland cotton (cv. TM-1). The Blast results showed that the three sequences are the nucleotide binding site-leucine rich repeat (NBS-LRR) type RGAs. Clustering results indicated that they are homologous to these published RGAs. Thus, the three RGAs can definitely be confirmed as NBS-LRR class of RGAs in upland cotton. Using single chromosome microdissection technique, DNA libraries containing cotton RGAs were obtained. This technique can promote cotton gene cloning, marker development and even the improvement of cotton genome research and breeding.

References

May 1, 1990·Human Genetics·G SengerU Claussen
Mar 1, 1994·Molecular & General Genetics : MGG·K ArumuganathanE D Earle
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·M VegaA A Levy
Mar 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S M SchmutzD C Winkelman
Nov 1, 1993·The Plant Journal : for Cell and Molecular Biology·D AlbaniL S Robert
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·V KanazinR C Shoemaker
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y G YuM A Maroof
Sep 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·T HoriS Mizuno
Nov 14, 1997·Genome Génome / Conseil National De Recherches Canada·C P ScuttP M Gilmartin
May 23, 1998·Journal of Medical Genetics·J J EngelenJ P Geraedts
Jul 22, 1998·The Plant Journal : for Cell and Molecular Biology·E SpeulmanJ L Beynon
Oct 13, 1998·Molecular Plant-microbe Interactions : MPMI·N C CollinsA Pryor
Jun 23, 2001·Mammalian Genome : Official Journal of the International Mammalian Genome Society·N SarkerH Yasue
Nov 10, 2001·Annual Review of Phytopathology·S H HulbertQ Sun
May 8, 2003·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Takahiro TaguchiKazunari Yuri
Aug 1, 1995·Genome Génome / Conseil National De Recherches Canada·Q Chen, K Armstrong
Aug 28, 2012·Nature Genetics·Kunbo WangShuxun Yu
Jun 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J SchondelmaierC Jung
Feb 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y KamisugiK Fukui
Sep 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K FukuiF Sakai
May 20, 2014·Nature Genetics·Fuguang LiShuxun Yu

❮ Previous
Next ❯

Datasets Mentioned

BETA
KU051681
KU051682
KU051680

Methods Mentioned

BETA
PCR
electrophoresis
Fluorescence
dissection

Software Mentioned

GeneBank
Phylip
BLAST
CellCut

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
Y Saitoh, J E Ikeda
Science in China. Series C, Life Sciences
X ZhengL Zhu
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Shu-Mei JiangZan-Min Hu
© 2022 Meta ULC. All rights reserved