Microdomains form on the luminal face of neuronal extracellular vesicle membranes.

Scientific Reports
Doreen MatthiesMaria S Ioannou

Abstract

Extracellular vesicles (EVs) are important mediators of cell-to-cell communication and have been implicated in several pathologies including those of the central nervous system. They are released by all cell types, including neurons, and are highly heterogenous in size and composition. Yet much remains unknown regarding the biophysical characteristics of different EVs. Here, using cryo-electron microscopy (cryoEM), we analyzed the size distribution and morphology of EVs released from primary cortical neurons. We discovered massive macromolecular clusters on the luminal face of EV membranes. These clusters are predominantly found on medium-sized vesicles, suggesting that they may be specific to microvesicles as opposed to exosomes. We propose that these clusters serve as microdomains for EV signaling and play an important role in EV physiology.

References

Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Oct 4, 2005·Journal of Structural Biology·Luis R Comolli, Kenneth H Downing
Nov 15, 2006·The Journal of Experimental Medicine·Michael E GreenbergStanley L Hazen
Jan 16, 2009·Trends in Cell Biology·Emanuele CocucciJacopo Meldolesi
May 5, 2012·Frontiers in Physiology·Carsten FrühbeisEva-Maria Krämer-Albers
Jun 5, 2012·Nature Cell Biology·Maria Francesca BaiettiGuido David
Sep 1, 2012·Nature Protocols·Gerard M J BeaudoinJyothi Arikkath
Dec 22, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Luca MusanteHarry Holthofer
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Jul 23, 2013·PLoS Biology·Carsten FrühbeisEva-Maria Krämer-Albers
Nov 14, 2013·Journal of Extracellular Vesicles·Rossella CrescitelliJan Lötvall
Mar 13, 2014·Journal of Thrombosis and Haemostasis : JTH·N ArraudA R Brisson
Mar 29, 2014·Journal of Extracellular Vesicles·Aleksander CvjetkovicCecilia Lässer
Nov 14, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lawrence RajendranXandra O Breakefield
Nov 14, 2015·Journal of Extracellular Vesicles·Johanna L Höög, Jan Lötvall
Dec 25, 2015·Journal of Extracellular Vesicles·Romain LinaresAlain R Brisson
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Joanna KowalClotilde Théry
Feb 14, 2016·Biochimica Et Biophysica Acta·Anna M JanasTadeusz Janas
Mar 5, 2016·Methods : a Companion to Methods in Enzymology·Rebecca F ThompsonNeil A Ranson
Jun 18, 2016·Journal of Structural Biology·Wim J H HagenJohn A G Briggs
Jul 23, 2016·Journal of Structural Biology·David N Mastronarde, Susannah R Held
Oct 21, 2016·Current Neuropharmacology·Allison L Isola, Suzie Chen
Mar 1, 2017·Nature Methods·UNKNOWN EV-TRACK ConsortiumAn Hendrix
Jul 19, 2017·Journal of Extracellular Vesicles·Davide ZabeoJohanna L Höög
Nov 29, 2017·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Anne-Lie StåhlDiana Karpman
Jan 18, 2018·Nature Reviews. Molecular Cell Biology·Guillaume van NielGraça Raposo
Mar 8, 2018·ELife·Timothy GrantNikolaus Grigorieff
Sep 6, 2018·The New England Journal of Medicine·Ravi ShahJane E Freedman
Mar 15, 2019·Emerging Microbes & Infections·Wenshuo ZhouHameeda Sultana
Mar 21, 2019·Cellular and Molecular Life Sciences : CMLS·Marta Monguió-TortajadaFrancesc E Borràs
Mar 30, 2019·Translational Psychiatry·Saumeh SaeediGustavo Turecki
Apr 3, 2019·Nature Cell Biology·Prabuddha SenguptaJennifer Lippincott-Schwartz
May 16, 2019·Proteomes·Magdalena SurmanMałgorzata Przybyło
Jul 19, 2019·PLoS Biology·Leonid Margolis, Yoel Sadovsky
May 2, 2019·BioRxiv : the Preprint Server for Biology·Jie E. YangEsther Bullitt

❮ Previous
Next ❯

Citations

Sep 25, 2020·The Journal of Membrane Biology·Maria S Ioannou
Dec 15, 2020·Journal of Chromatography. a·Thanaporn LiangsupreeMarja-Liisa Riekkola

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
transmission electron microscopy
electron tomography
electron microscopy

Key Resources (RRID) Mentioned

AB_2313773
AB_445200
AB_398140

Software Mentioned

SerialEM
SPSS Statistics
IMOD
ImageJ
cisTEM
ZEN
CryoEM
EV
GraphPad Prism
TRACK

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.