Microdroplet fluorochromatic assay for the enumeration of white cells (WBCs) in WBC-reduced blood components: validation and application for evaluating newly developed WBC-reduction filters

Transfusion
P Borzini, L Dumont

Abstract

Sensitive and accurate counting methods are required to assess the residual white cells (WBCs) in WBC-reduced blood components. The Nageotte hemocytometer, widely used for this purpose, is cumbersome, and its efficacy is dependent upon the skill of the operator. The performance of a simple fluorochromatic assay using tissue-typing microdroplet trays is presented here. Undiluted samples of blood components were mixed with a fluorochromatic dye in trays. WBCs were counted under an epifluorescence microscope. The accuracy and sensitivity of this method were compared with those of the reference Nageotte hemocytometer method by using serial dilution of samples of platelets and red cells containing known concentrations of WBCs and by calculating the standard curves. The Nageotte hemocytometer and the microdroplet fluorochromatic assay (MFA) were also compared in terms of count correlation and reproducibility in 320 paired counts of plateletpheresis samples. MFA was used to evaluate a newly developed WBC-reduction red cell filter. The MFA for platelets and red cells was linear to 0.1 and 0.03 WBCs per microL, respectively. The linear regression line of log10 MFA versus log10 Nageotte method had a slope of 0.963, intercept of -0.04, an...Continue Reading

Citations

Jun 3, 1999·Transfusion Science·R N PieterszM J Seghatchian
May 13, 1998·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·J A Loos, J L Wautier
Jun 27, 2003·Cytometry. Part B, Clinical Cytometry·M J Dijkstra-TiekstraJ de Wildt-Eggen
Dec 28, 2007·The Korean journal of laboratory medicine·Sook Won Ryu, In Bum Suh

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