MicroED: a versatile cryoEM method for structure determination.

Emerging Topics in Life Sciences
Brent L Nannenga, Tamir Gonen

Abstract

Micro-electron diffraction, or MicroED, is a structure determination method that uses a cryo-transmission electron microscope to collect electron diffraction data from nanocrystals. This technique has been successfully used to determine the high-resolution structures of many targets from crystals orders of magnitude smaller than what is needed for X-ray diffraction experiments. In this review, we will describe the MicroED method and recent structures that have been determined. Additionally, applications of electron diffraction to the fields of small molecule crystallography and materials science will be discussed.

References

Oct 15, 1975·Journal of Molecular Biology·P N Unwin
May 25, 1975·Journal of Molecular Biology·P N Unwin, R Henderson
Jul 21, 1972·Science·V R MatricardiD F Parsons
Jan 1, 1981·Annual Review of Biophysics and Bioengineering·J DubochetC A Walter
Aug 29, 2003·Acta Crystallographica. Section A, Foundations of Crystallography·J S Wu, J C H Spence
Sep 27, 2005·Journal of Structural Biology·David N Mastronarde
Apr 9, 1937·Science·J B Sumner, A L Dounce
Dec 15, 2007·Nature Protocols·Robert A GrassucciJoachim Frank
Oct 4, 2008·Journal of the American Chemical Society·Ronald D WamplerGarth J Simpson
May 2, 2009·Nature·Junliang SunXiaodong Zou
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Feb 5, 2011·Nature·Henry N ChapmanJohn C H Spence
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·T Geoff G BattyeAndrew G W Leslie
Jun 12, 2012·Angewandte Chemie·Enrico MugnaioliUte Kolb
Nov 8, 2012·Methods in Molecular Biology·Tamir Gonen
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Raquel Martínez-FrancoAvelino Corma
Jun 26, 2013·Acta Crystallographica. Section D, Biological Crystallography·Igor NederlofJan Pieter Abrahams
Jul 19, 2013·Biochimica Et Biophysica Acta·Isabel MoraesPatrick D Shaw Stewart
Oct 23, 2013·Journal of the American Chemical Society·Yue-Biao ZhangOmar M Yaghi
Dec 21, 2013·Science·Wei LiuVadim Cherezov
Apr 9, 2014·Current Opinion in Structural Biology·Brent L Nannenga, Tamir Gonen
May 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Hilary P StevensonGuillermo Calero
Oct 11, 2014·ELife·Brent L NannengaTamir Gonen
Mar 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Koji YonekuraChikashi Toyoshima
Jul 2, 2015·Acta Crystallographica. Section A, Foundations and Advances·Johan HattneTamir Gonen
Sep 10, 2015·Nature·Jose A RodriguezDavid S Eisenberg
Mar 6, 2016·Archives of Biochemistry and Biophysics·Christopher O BarnesGuillermo Calero
Apr 15, 2016·Nature Protocols·Dan ShiTamir Gonen
May 4, 2016·Acta Crystallographica. Section D, Structural Biology·Hilary P StevensonGuillermo Calero
Jun 9, 2016·Journal of Applied Crystallography·Johan HattneTamir Gonen
Jul 28, 2016·Protein Science : a Publication of the Protein Society·Shian LiuTamir Gonen
Sep 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Michael R SawayaDavid S Eisenberg
Oct 5, 2016·Current Opinion in Structural Biology·Brent L Nannenga, Tamir Gonen
Jun 27, 2017·Current Opinion in Structural Biology·Jose A RodriguezTamir Gonen
Sep 7, 2017·Acta Crystallographica. Section D, Structural Biology·M T B ClabbersJ P Abrahams
Oct 27, 2017·The Journal of Physical Chemistry Letters·Sandra VergaraGuillermo Calero

❮ Previous
Next ❯

Citations

Oct 8, 2019·Acta Crystallographica. Section D, Structural Biology·Dorothee LiebschnerPaul D Adams
Sep 12, 2019·Frontiers in Molecular Biosciences·Angel Rivera-Calzada, Marta Carroni
Sep 29, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Krishna P KhakurelJakob Andreasson
Nov 4, 2020·Molecular Microbiology·Trishant R UmrekarMorgan Beeby
Jan 19, 2021·ACS Omega·Esma Khatun, Thalappil Pradeep
Oct 24, 2020·Current Pathobiology Reports·Rachel CarterAmanda Haymond
May 13, 2019·Trends in Biochemical Sciences·Radostin DanevMasahide Kikkawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Current Opinion in Structural Biology
Brent L Nannenga, Tamir Gonen
Current Opinion in Colloid & Interface Science
Michael W Martynowycz, Tamir Gonen
Current Opinion in Structural Biology
Brent L Nannenga, Tamir Gonen
Proceedings of the National Academy of Sciences of the United States of America
Michael R SawayaDavid S Eisenberg
© 2022 Meta ULC. All rights reserved