Microelastic properties of lung cell-derived extracellular matrix.

Acta Biomaterialia
Patricia A SoucyLewis H Romer

Abstract

The mechanical properties of the extracellular microenvironment regulate cell behavior, including migration, proliferation and morphogenesis. Although the elastic moduli of synthetic materials have been studied, little is known about the properties of naturally produced extracellular matrix. Here we have utilized atomic force microscopy to characterize the microelastic properties of decellularized cell-derived matrix from human pulmonary fibroblasts. This heterogeneous three-dimensional matrix had an average thickness of 5 ± 0.4 μm and a Young's modulus of 105 ± 14 Pa. Ascorbate treatment of the lung fibroblasts prior to extraction produced a twofold increase in collagen I content, but did not affect the stiffness of the matrices compared with matrices produced in standard medium. However, fibroblast-derived matrices that were crosslinked with glutaraldehyde demonstrated a 67% increase in stiffness. This work provides a microscale characterization of fibroblast-derived matrix mechanical properties. An accurate understanding of native three-dimensional extracellular microenvironments will be essential for controlling cell responses in tissue engineering applications.

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Citations

Jul 18, 2012·Proceedings of the American Thoracic Society·Boris Hinz
Mar 6, 2012·The Annals of Thoracic Surgery·Dhruva K MishraMin P Kim
Oct 7, 2015·Methods : a Companion to Methods in Enzymology·Marta TelloJacky G Goetz
Sep 16, 2014·Acta Biomaterialia·Patricia A SoucyLewis Romer
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May 11, 2016·Journal of Cellular Physiology·Ignasi JorbaDaniel Navajas
Jul 27, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Fumin ChangLewis Romer
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Mar 1, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Artem ShkumatovHyunjoon Kong
Nov 16, 2019·Cancer Cell International·Dennis KeurhorstChristian Stock

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