PMID: 6408601Apr 1, 1983Paper

Microelectrode measurement of intracellular chloride activity in smooth muscle cells of guinea-pig ureter

Pflügers Archiv : European journal of physiology
C C Aickin, N A Vermuë

Abstract

The intracellular Cl activity (aiCl) of smooth muscle cells in the guinea-pig ureter was measured using double-barrelled Cl-sensitive microelectrodes. The mean aiCl in normal Krebs solution was 51.1 mM, equivalent to an ECl of -18.6 mV, with a mean Em of -48.7 mV. Thus aiCl was three times higher than predicted from a passive distribution, aiCl exceeded the extracellular activity by nearly 10 mM in 10%-Cl solution. Complete removal of extracellular Cl (Cl0) caused a decline in aiCl to an apparent level of 3.3 mM with a mean time constant of 6.7 min. Cl ions were reaccumulated against their electrochemical gradient on readmission of Cl0 with a mean time constant of 6.6 min. Both the reaccumulation and loss of Cl ions on changing Cl0 were slowed about three-fold by the presence of the anion exchange inhibitor DIDS. It is therefore concluded that most of the transmembrane Cl movements are mediated by a reversible anion exchange carrier and that PCl is very low. These results are similar to those obtained in vas deferens and may be a general feature of smooth muscle cells.

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Citations

Jun 1, 1992·Pflügers Archiv : European journal of physiology·P PacaudJ Mironneau
Apr 1, 1989·Pflügers Archiv : European journal of physiology·P PacaudJ Mironneau
Dec 12, 1996·European Journal of Pharmacology·A J KirkupA H Weston
Feb 28, 2001·Progress in Biophysics and Molecular Biology·A R Chipperfield, A A Harper
Jun 5, 1998·Clinical and Experimental Pharmacology & Physiology·R J LangM F Klemm
Oct 1, 2013·Pflügers Archiv : European journal of physiology·Simon Bulley, Jonathan H Jaggar
Oct 1, 1993·Pflügers Archiv : European journal of physiology·C Van Renterghem, M Lazdunski
Nov 16, 2014·The Journal of Physiology·Xin JinNikita Gamper
Dec 24, 2005·Autonomic & Autacoid Pharmacology·D BiegerR Tabrizchi
Jan 16, 2015·Pflügers Archiv : European journal of physiology·Christian A HübnerHeimo Ehmke
Jun 5, 2001·Japanese Journal of Pharmacology·K Kitamura, J Yamazaki
Mar 2, 1999·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C B KölbelE A Mayer
Dec 8, 2005·Canadian Journal of Physiology and Pharmacology·Luis M Montaño, Blanca Bazán-Perkins
Nov 1, 1989·World Journal of Surgery·L DogliottiA Angeli
Feb 5, 2002·Canadian Journal of Physiology and Pharmacology·J JuryE E Daniel
Sep 5, 1998·The American Journal of Physiology·L J JanssenR Nana
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Luke J Janssen
Mar 1, 1988·The American Journal of Physiology·H YamaguchiF S Fay
Feb 5, 2000·Progress in Neurobiology·S FringsS J Kleene

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