Microencapsulated bile salt hydrolase producing Lactobacillus reuteri for oral targeted delivery in the gastrointestinal tract

Applied Microbiology and Biotechnology
Christopher MartoniSatya Prakash

Abstract

This is the first study of its kind to screen probiotic lactic acid bacteria for the purpose of microencapsulating a highly bile salt hydrolase (BSH)-active strain. A Lactobacillus reuteri strain and a Bifidobacterium longum strain were isolated as the highest BSH producers among the candidates. Microcapsules were prepared with a diameter of 619 +/- 31 mum and a cell load of 5 x 10(9) cfu/ml. Post de Man, Rogosa, and Sharpe broth-acid challenge, L. reuteri microcapsules metabolized glyco- and tauro-conjugated bile salts at rates of 10.16 +/- 0.46 and 1.85 +/- 0.33 micromol/g microcapsule per hour, respectively, over the first 2 h. Microencapsulated B. longum had minimal BSH activity and were significantly (P < 0.05) more susceptible to acid challenge. Further testing of L. reuteri microcapsules in a simulated human gastrointestinal (GI) model showed an improved rate, with 49.4 +/- 6.21% of glyco-conjugates depleted after 60 min and complete deconjugation after 4 h. Microcapsules protected the encased cells in the simulated stomach maintaining L. reuteri viability above 10(9), 10(8), and 10(6) cfu/ml after 2 h at pH 3.0, 2.5, and 2.0, respectively. Results show excellent potential for this highly BSH-active microencapsulation sy...Continue Reading

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Feb 15, 2008·Journal of Biomedicine & Biotechnology·Christopher MartoniSatya Prakash

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Citations

Aug 12, 2009·Applied Biochemistry and Biotechnology·Anil K PatelSudhir B Chincholkar
Aug 19, 2011·Biologics : Targets & Therapy·Satya PrakashCatherine Tomaro-Duchesneau
Sep 13, 2013·BioMed Research International·Himanshu K SolankiPrachi M Thakar
Jul 21, 2011·Journal of Biomedicine & Biotechnology·Satya PrakashArielle Cantor
Feb 22, 2012·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Nathan J Rogers, Shaker A Mousa
Aug 6, 2015·Journal of Food Science and Technology·Siavash IravaniSeyed Vahid Mirmohammadi
Mar 20, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mitchell L JonesSatya Prakash
Dec 18, 2008·FEMS Microbiology Letters·Joel Schroeter, Todd Klaenhammer
Jul 28, 2016·International Journal of Food Sciences and Nutrition·Chaline Caren CoghettoMarco Antônio Záchia Ayub
Aug 15, 2012·Artificial Cells, Nanomedicine, and Biotechnology·Laetitia RodesSatya Prakash
Jan 27, 2010·Current Opinion in Supportive and Palliative Care

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