Microencapsulated Schwann cell transplantation inhibits P2X3 receptor expression in dorsal root ganglia and neuropathic pain

Neural Regeneration Research
Ya-Ling ZhangZeng-Xu Liu

Abstract

Schwann cell transplantation is a promising method to promote neural repair, and can be used for peripheral nerve protection and myelination. Microcapsule technology largely mitigates immune rejection of transplanted cells. We previously showed that microencapsulated olfactory ensheathing cells can reduce neuropathic pain and we hypothesized that microencapsulated Schwann cells can also inhibit neuropathic pain. Rat Schwann cells were cultured by subculture and then microencapsulated and were tested using a rat chronic constriction injury (CCI) neuropathic pain model. CCI rats were treated with Schwann cells or microencapsulated Schwann cells and were compared with sham and CCI groups. Mechanical withdrawal threshold and thermal withdrawal latency were assessed preoperatively and at 1, 3, 5, 7, 9, 11 and 14 days postoperatively. The expression of P2X3 receptors in L4-5 dorsal root ganglia of the different groups was detected by double-label immunofluorescence on day 14 after surgery. Compared with the chronic constriction injury group, mechanical withdrawal threshold and thermal withdrawal latency were higher, but the expression of P2X3 receptors was remarkably decreased in rats treated with Schwann cells and microencapsulated ...Continue Reading

References

Feb 27, 2007·Brain, Behavior, and Immunity·Wendy Marie Campana
Sep 23, 2008·Glia·John W Griffin, Wesley J Thompson
Aug 31, 2010·Brain : a Journal of Neurology·Timothy K Y KaanStephen B McMahon
May 8, 2014·Journal of Materials Science. Materials in Medicine·Antos ShakhbazauRajiv Midha
Dec 2, 2014·Scientific Reports·Qing ZhuJun-Xu Li
Dec 17, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Jian-Qing LinXian-Zhong Lin
Jun 2, 2015·Tissue Engineering. Part B, Reviews·Haitao ZhuBo Wang
Jan 29, 2016·Cell Transplantation·Stanislava JergovaJacqueline Sagen
Feb 3, 2016·Brain Research·Paulino Barragán-IglesiasVinicio Granados-Soto
May 28, 2016·Molecular Brain·Yung-Hui Kuan, Bai-Chuang Shyu
Aug 12, 2016·Drugs & Aging·Gisèle PickeringBernard Wary
Oct 16, 2016·Methods in Molecular Biology·Masayuki Shimoda, Shinichi Matsumoto
Nov 11, 2016·Methods in Molecular Biology·Raphael P H MeierLeo H Bühler
Dec 9, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amit MoghaKelly R Monk
Feb 14, 2017·Neurochemistry International·Mofeng YingShuangmei Liu
Feb 18, 2017·Journal of Materials Science. Materials in Medicine·Antonio MerolliJoachim Kohn
Mar 23, 2017·ACS Chemical Neuroscience·Young-Hwan JungYong-Chul Kim
Apr 14, 2017·The Journal of Pharmacology and Experimental Therapeutics·Yevheniia IshchenkoRashid Giniatullin
Sep 15, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jose A Gomez-SanchezKristjan R Jessen
Oct 3, 2017·Neural Regeneration Research·Susanne Quintes, Bastian G Brinkmann
Dec 13, 2017·International Journal of Biological Sciences·Yang WangZunli Shen
Dec 23, 2017·International Journal of Molecular Sciences·Cen-Hung LinShu-Hung Huang
Dec 24, 2017·Neuroscience·B SagalajevA Pertovaara
Jan 24, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Zhiyong WangWeiguang Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
transmission electron microscopy

Software Mentioned

Image
Pro Plus
SPSS

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.