Microencapsulation of β-galactosidase with different biopolymers by a spray-drying process

Food Research International
Berta N EstevinhoFernando Rocha

Abstract

The aim of this work was to investigate the possibility of producing microparticles containing β-galactosidase, using different biopolymers (arabic gum, chitosan, modified chitosan, calcium alginate and sodium alginate) as encapsulating agents by a spray-drying process. This study focused on the enzyme β-galactosidase, due to its importance in health and in food processing. Encapsulation of β-galactosidase can increase the applicability of this enzyme in different processes and applications. A series of β-galactosidase microparticles were prepared, and their physicochemical structures were analyzed by laser granulometry analysis, zeta potential analysis, and by scanning electron microscopy (SEM). Microparticles with a mean diameter around 3μm have been observed, for all the biopolymers tested. The microparticles formed with chitosan or arabic gum presented a very rough surface; on the other hand, the particles formed with calcium or sodium alginate or modified chitosan presented a very smooth surface. The activity of the enzyme was studied by spectrophotometric methods using the substrate ONPG (O-nitrophenyl-β,d-galactopyranoside). The microencapsulated β-galactosidase activity decreases with all the biopolymers. The relative e...Continue Reading

Citations

Dec 8, 2015·Biological Trace Element Research·Kimmi MukhijaNitin Kumar Singhal
Mar 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sarah L Perry, David Julian McClements
Nov 28, 2015·Journal of Microencapsulation·Francisca Casanova, Lúcia Santos
May 10, 2017·Biomacromolecules·Maria Victoria Traffano-SchiffoPatricio R Santagapita

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