Microencapsulation of Lactobacillus acidophilus La-05 and incorporation in vegan milks: Physicochemical characteristics and survival during storage, exposure to stress conditions, and simulated gastrointestinal digestion

Food Research International
Laênia Angélica Andrade LopesThayza Christina Montenegro Stamford

Abstract

The effect of microencapsulation of L. acidophilus La-05 (8 log CFU/mL) by external ionic gelation technique in alginate (30 g/L; AM) and alginate coated with a low molecular weight chitosan solution (5 g/L; AC5M) on the survival of the freeze-dried probiotic culture during storage (7 °C; 0, 7, 15, 30, 60, 90 and 120 days), and exposure to temperature (72, 85 and 90 °C), pH (2, 4, and 6), and NaCl (10, 15 and 20 g/L) were studied. Furthermore, vegan milks (soybean and rice milks) added with microencapsulated probiotic cultures were evaluated for the physicochemical characteristics and survival of the probiotic culture during refrigerated storage (7 °C; 7 days) and in vitro digestion. Free cells were used as control. AM and AC5M showed similar microencapsulation yield (>90%) with uniform and spherical microparticles dispersed without agglomeration. Scanning electron microscopy showed that chitosan was able to cover the porous structure of the alginate particles, resulting in a more stabilized microparticle. The microencapsulation provided higher probiotic protection to storage, thermal treatment, NaCl and pH (decreases of ~1 log CFU/mL) compared to the free cells (decreases of ~3, 4, 2 and 3 log CFU/mL, respectively), and increa...Continue Reading

References

Feb 13, 2001·Journal of Microbiological Methods·B HerigstadJ Heersink
Jul 28, 2010·International Journal of Food Microbiology·María ChávarriMaría del Carmen Villarán
Sep 4, 2012·Carbohydrate Polymers·Sawaminee NualkaekulDimitris Charalampopoulos
Jul 16, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Tao JiangYun-Jaie Choi
Sep 1, 2015·Food Research International·Marta AlvesF Xavier Malcata
Apr 10, 2018·Critical Reviews in Food Science and Nutrition·Min MinMalik A Hussain
Jul 7, 2018·Journal of Food and Drug Analysis·Rout George KerryGitishree Das

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