Microencapsulation of Live Cells in Synthetic Polymer Capsules

ACS Omega
Reza Roghani EsfahaniJoerg Lahann

Abstract

In cell therapies, it is advantageous to encapsulate live cells in protective, semipermeable microparticles for controlled release of cytokines, growth factors, monoclonal antibodies, or insulin. Here, a modified electrospraying approach with an organic solution of poly(lactide-co-glycolide) (PLGA) polymer is used to create synthetic PLGA capsules that effectively protect live cells. Using a design of experiment (DOE) methodology, the effect of governing jetting parameters on encapsulation efficiency, yield, and size is systematically evaluated. On the basis of this analysis, the interaction between bovine serum albumin concentration and core flow rate is the most dominant factor determining core encapsulation efficiency as well as the microcapsule size. However, the interaction between shell solvent ratio and shell flow rate predominantly defines the particle yield. To validate these findings, live cells have been successfully encapsulated in microcapsules using optimized parameters from the DOE analysis and have survived the electrohydrodynamic jetting process. Extending the currently available toolkit for cell microencapsulation, these biodegradable, semi-impermeable cell-laden microcapsules may find a range of applications ...Continue Reading

References

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Citations

Feb 29, 2020·Biomaterials Science·Barbara Kupikowska-Stobba, Dorota Lewińska
Feb 7, 2018·Advanced Healthcare Materials·Asish C Misra, Joerg Lahann
Jun 20, 2019·Frontiers in Bioengineering and Biotechnology·Shuixan Hu, Paul de Vos
Jul 4, 2021·Advanced Healthcare Materials·Maria C GomesJoão F Mano
Aug 3, 2021·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Cleo ParisiFrancisco M Fernandes
Jan 11, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Takaichi WatanabeTsutomu Ono
Oct 2, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Saahil ShethSilviya P Zustiak

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Methods Mentioned

BETA
biosensor
flow cytometry
scanning electron microscopy
chip
confocal microscopy

Software Mentioned

DOE
Minitab

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