Microenvironmental changes induced by placenta-derived mesenchymal stem cells restore ovarian function in ovariectomized rats via activation of the PI3K-FOXO3 pathway.

Stem Cell Research & Therapy
Jong Ho ChoiGi Jin Kim

Abstract

Translational studies have explored the therapeutic potential and feasibility of mesenchymal stem cells (MSCs) in several degenerative diseases; however, mechanistic studies of the function of these cells have been insufficient. As ovarian failure causes anovulation as well as ovarian steroid hormonal imbalances, the specific aims of this study were to analyze the therapeutic role of placenta-derived MSCs (PD-MSCs) in an ovarian failure ovariectomy (OVX) rat model and evaluate whether PD-MSC transplantation (Tx) improved folliculogenesis and oocyte maturation in the injured ovary through PI3K/Akt and FOXO signaling. Blood and ovary tissue were collected and analyzed after various PD-MSC Tx treatments in an ovariectomized rat model. Changes in the expression of folliculogenesis- and ovary regeneration-related genes induced by PD-MSC treatments were analyzed by qRT-PCR, Western blotting, and histological analysis. The levels of hormones related to ovary function were significantly increased in the PD-MSC Tx groups compared with those in the nontransplantation group (NTx). The follicle numbers in the ovarian tissues were increased along with the increased expression of genes related to folliculogenesis in the PD-MSC Tx groups comp...Continue Reading

References

Aug 25, 2004·Science·Aleksandar RajkovicMartin M Matzuk
May 19, 2005·Treatments in Endocrinology·Madelon van WelyFulco van der Veen
Jan 19, 2007·Nature Clinical Practice. Cardiovascular Medicine·Ramesh Mazhari, Joshua M Hare
Aug 1, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ho-Joon LeeJonathan L Tilly
Jan 19, 2008·Climacteric : the Journal of the International Menopause Society·Nick Panay, Anna Fenton
Feb 23, 2008·Science·Srinivas R ViswanathanRichard I Gregory
May 24, 2008·Human Reproduction Update·Stephen FranksKate Hardy
May 30, 2008·Biology of Reproduction·Youngsok ChoiAleksandar Rajkovic
Jul 8, 2008·Developmental Biology·George B JohnDiego H Castrillon
Oct 28, 2008·Molecular Cell·Inha HeoV Narry Kim
Mar 5, 2009·Biochemical and Biophysical Research Communications·Hong LiuRen-Li Zhang
Jul 24, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Xu-Xia SuiYu-Cai Fu
Mar 25, 2011·Methods in Molecular Biology·Nina IlicKerry Atkinson
May 31, 2011·Frontiers in Bioscience (Landmark Edition)·Ingrid U SchraufstatterSophia Khaldoyanidi
Aug 4, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Nina Henriette Uhlenhaut, Mathias Treier
Jan 14, 2012·Molecular and Cellular Endocrinology·Ranna El-KhairiJohn C Achermann
Dec 6, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yuji TakeharaOsamu Kato
Apr 9, 2013·Cell Stem Cell·Ng Shyh-Chang, George Q Daley
Oct 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kazuhiro KawamuraAaron J Hsueh
Jul 22, 2014·Respirology : Official Journal of the Asian Pacific Society of Respirology·Daniel C ChambersPeter M A Hopkins
Aug 12, 2014·Vojnosanitetski pregled. Military-medical and pharmaceutical review·Nina Ilić, Kerry Atkinson
Dec 3, 2014·The Medical Clinics of North America·Subbulaxmi Trikudanathan
Jun 17, 2015·BMC Biology·Yu RenAleksandar Rajkovic
Sep 10, 2015·Cellular and Molecular Life Sciences : CMLS·Beate HeissigKoichi Hattori

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Citations

Apr 10, 2021·Reproductive Sciences·P PetricM Stimpfel
Aug 17, 2021·Journal of Clinical Laboratory Analysis·Haiwei WangLiangpu Xu

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Methods Mentioned

BETA
hormone replacement therapy
ELISA
electrophoresis
light microscopy
enzyme-linked immunosorbent assays
enzyme-linked immunosorbent assay
nuclear translocation

Software Mentioned

SAS
ImageJ

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