Microextraction in packed sorbent for analysis of antidepressants in human plasma by liquid chromatography and spectrophotometric detection

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Andréa R ChavesMaria Eugênia C Queiroz

Abstract

A sensitive and reproducible method by microextraction packed sorbent and liquid chromatography with UV detection (MEPS/LC-UV) is described for the determination of new generation antidepressants (sertraline, mirtazapine, fluoxetine, citalopram and paroxetine) in human plasma samples. The MEPS variables, such as sample volume, pH, number of extraction cycles (draw-eject), and desorption conditions (solvent and solvent volume of elution) influenced the MEPS/LC efficiency significantly. Important factors in the optimization of MEPS efficiency, as well as washing steps and carryover effect are discussed. The analyses were carried out using small sample volumes (400 microL), and in a short time period (3 min for the entire sample preparation step). The MEPS/LC-UV method was shown to be linear at concentrations ranging from the limit of quantification (LOQ) to 1000 ng mL(-1). The LOQ values ranged from 10 to 25 ng mL(-1). The inter-day precision of the method presented coefficient of the variation ranging from 1.3% to 8.7%. On the basis of analytical validation, it is shown that the MEPS/LC-UV methodology is adequate for antidepressant analysis, from therapeutic to toxic levels. In order to evaluate the proposed method for clinical ...Continue Reading

References

Feb 3, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marina VitaMohamed Abdel-Rehim
Apr 4, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Roberto MandrioliMaria Augusta Raggi

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Citations

Oct 18, 2011·Analytical and Bioanalytical Chemistry·Renata Wietecha-PosłusznyPaweł Kościelniak
Apr 2, 2014·Journal of Analytical Methods in Chemistry·Mohammad Mahdi MoeinMohamed Abdel-Rehim
May 23, 2012·Bioanalysis·Christopher Singleton
Aug 11, 2012·Bioanalysis·Mário BarrosoEugenia Gallardo
May 2, 2014·Journal of Pharmaceutical and Biomedical Analysis·Iara Maíra de Oliveira VianaChristian Fernandes
Oct 9, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ivo MorenoEugenia Gallardo
Jul 7, 2012·Biomedical Chromatography : BMC·Khalil FarhadiAmir Abbas Matin
Dec 15, 2010·Biomedical Chromatography : BMC·Prashant Laxman KoleRavi Sheshala
Nov 30, 2012·Journal of Chromatographic Science·Fernanda H Salami, Maria Eugênia C Queiroz
Oct 5, 2018·Critical Reviews in Analytical Chemistry·M T García-CórcolesA Zafra-Gómez
May 15, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Zeynab KhoshdelAlireza Badiei
Sep 22, 2012·Journal of Separation Science·Esme CandishEmily F Hilder
May 31, 2017·Bulletin of Environmental Contamination and Toxicology·Elif Seda KoçoğluSeyfullah Keyf
May 6, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Artemis LioupiVictoria Samanidou

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