DOI: 10.1101/509265Jan 2, 2019Paper

Microextrusion Printing Cell-Laden Networks of Type I Collagen with Patterned Anisotropy and Geometry

BioRxiv : the Preprint Server for Biology
Bryan NergerCeleste Nelson


Type I collagen self-assembles into three-dimensional (3D) fibrous networks. These dynamic viscoelastic materials can be remodeled in response to mechanical and chemical cues to form anisotropic networks, the structure of which influences tissue development, homeostasis, and disease progression. Conventional approaches for fabricating anisotropic networks of type I collagen are often limited to unidirectional alignment over small areas. Here, we describe a new approach for engineering cell-laden anisotropic networks of type I collagen fibers using 3D microextrusion printing of a collagen-Matrigel ink. By adding molecular crowders, we demonstrate hierarchical control of 3D-printed collagen with the ability to spatially pattern collagen fiber anisotropy and geometry. Our data suggest that collagen anisotropy results from a combination of molecular crowding in the ink and shear and extensional flows present during 3D-printing. We demonstrate that human breast cancer cells cultured on 3D-printed collagen orient along the direction of collagen fiber alignment. We also demonstrate the ability to simultaneously bioprint epithelial cell clusters and control the alignment and geometry of collagen fibers surrounding cells in the bioink. ...Continue Reading

Related Concepts

Epithelial Cells
Visual Perception
Disease Progression
Fat-laden Macrophage
Laboratory Culture

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.