Microfabricated biosensor for the simultaneous amperometric and luminescence detection and monitoring of Ochratoxin A

Biosensors & Bioelectronics
S A TriaAnthony Guiseppi-Elie

Abstract

The low molecular weight hapten, Ochratoxin A (OTA), is a natural carcinogenic mycotoxin produced by Aspergillus and Penicillium fungi and so it commonly appears in wines, other foods, and in the environment. An amperometric biosensor has been developed that uses the immobilized synthetic peptide, NFO4; which possesses a high binding affinity and thus provides for molecular recognition of OTA; simulating the mycotoxin-specific antibody. Biotransducers were produced from a microlithographically fabricated electrochemical cell-on-a-chip that uses the microdisc electrode array working electrode format augmented with microporous graphitized carbon (MGC) that was electrodeposited within a poly(aniline-co-meta-aminoaniline) electroconductive polymer layer. A redox mediator, iron-nickel hexacyanoferrate (Fe|NiHCF) was amperometrically deposited onto the MGC. The device was then dip-coated with monomer cocktail that yielded poly(HEMA-co-AEMA) foam that was prepared in-situ by UV crosslinking and by sequentially freezing followed by freeze drying of the chip to yield a 3-D support for the chelation of Zn(2+) ions (ZnCl2) and the subsequent immobilization of N-terminus his-tagged peptide, NFO4. To conduct the biosensors assay, HRP conjug...Continue Reading

References

Jan 1, 1995·Natural Toxins·J E SmithJ G Anderson
Mar 4, 2005·Critical Reviews in Toxicology·Evelyn O'Brien, Daniel R Dietrich
Dec 8, 2005·Food Additives and Contaminants·Kevin Jørgensen
Nov 24, 2007·Biosensors & Bioelectronics·Beatriz Prieto-SimónThierry Noguer
Aug 5, 2008·Biomedical Microdevices·Abdur Rub Abdur RahmanAnthony Guiseppi-Elie
Oct 25, 2008·Analytical Chemistry·Jorge A Cruz-Aguado, Gregory Penner
Nov 6, 2008·Journal of Agricultural and Food Chemistry·Jorge A Cruz-Aguado, Gregory Penner
Nov 18, 2008·Biosensors & Bioelectronics·Abd-Elgawad RadiJean-Louis Marty
May 22, 2010·Journal of Chromatography. a·Wassim Hadj AliValérie Pichon
Oct 22, 2010·Analytical and Bioanalytical Chemistry·Anthony Guiseppi-Elie
Feb 26, 2011·Analytical Chemistry·Natalya A SitnikovaArkady A Karyakin
Jun 1, 2010·Toxins·Jorn C C Yu, Edward P C Lai
Aug 14, 2012·Biosensors & Bioelectronics·I BazinC Gonzalez
Dec 4, 2012·Talanta·Christian N KotanenAnthony Guiseppi-Elie

❮ Previous
Next ❯

Citations

Jul 29, 2017·Journal of Chemical Information and Modeling·Aby A ThyparambilAnthony Guiseppi-Elie
Aug 2, 2017·Engineering in Life Sciences·Harshvardhan ModhJohanna-Gabriela Walter
May 16, 2017·Toxins·Aby A ThyparambilAnthony Guiseppi-Elie
Nov 12, 2017·Analytical and Bioanalytical Chemistry·Riikka PeltomaaMaría C Moreno-Bondi
Aug 16, 2017·Polymers·Javad Tavakoli, Youhong Tang
Dec 29, 2020·Biosensors & Bioelectronics·John R AggasAnthony Guiseppi-Elie
Mar 27, 2021·Annual Review of Food Science and Technology·Hazal Turasan, Jozef Kokini
Apr 4, 2021·Sensors·Ricardo Matias TrujilloRossana Elena Madrid

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.