Microfabricated differential mobility spectrometry with pyrolysis gas chromatography for chemical characterization of bacteria

Analytical Chemistry
Hartwig SchmidtG A Eiceman

Abstract

A microfabricated drift tube for differential mobility spectrometry (DMS) was used with pyrolysis-gas chromatography (py-GC) to chemically characterize bacteria through three-dimensional plots of ion intensity, compensation voltage from differential mobility spectra, and chromatographic retention time. The DMS analyzer provided chemical information for positive and negative ions simultaneously from chemical reactions between pyrolysis products in the GC effluent and reactant ions of H+(H2O)n and O2-(H2O)n in air at ambient pressure. Authentic standards for chemicals formed in the pyrolysis of bacteria showed favorable matches with plots from py-GC/DMS analysis and were supported by py-GC/MS results. These and other yet-unidentified constituents provided a means to distinguish Escherichia coli from Micrococcus luteus. A Gram-positive spore former (Bacillus megaterium) was distinguished by an abundant peak for crotonic acid evident in positive and negative ions and not observed with M. luteus. In contrast, plots from py-GC/DMS of lipid A and lipoteichoic acid showed poor matches to plots for a Gram-negative (E. coli) bacterium and a Gram-positive (M. luteus) bacterium and the differences were attributed to differences in genus so...Continue Reading

References

Jul 29, 1999·Analytical Chemistry·P A DemirevC Fenselau
May 7, 2003·Journal of Microbiological Methods·Jeffrey S Buyer

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Citations

Mar 31, 2009·Analytical and Bioanalytical Chemistry·Sasidhar MaddulaJörg Ingo Baumbach
Aug 29, 2007·Journal of the American Society for Mass Spectrometry·Alexandre A Shvartsburg, Richard D Smith
Jul 1, 2008·Journal of the American Society for Mass Spectrometry·Alexandre A Shvartsburg, Richard D Smith
Jun 2, 2006·Analytical Chemistry·Alexandre A ShvartsburgRichard D Smith
Sep 30, 2009·Analytical Chemistry·Satendra PrasadRichard D Smith
Aug 28, 2009·Analytical Chemistry·Alexandre A ShvartsburgDanielle Toutoungi
May 5, 2007·The Review of Scientific Instruments·Alexey AdamovTapio Kotiaho
Mar 24, 2016·Analytical and Bioanalytical Chemistry·Lena Ganda SaptalenaUrsula Telgheder
Dec 11, 2007·Journal of Chromatography. a·Abu B Kanu, Herbert H Hill
Jan 16, 2010·Journal of Applied Physiology·Wolfgang VautzMarcus Peters
Apr 9, 2009·Analytical Chemistry·Adarsh D RadadiaMark A Shannon
Nov 16, 2006·Analytical Chemistry·Erkinjon G NazarovGary A Eiceman

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