Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro

The International Journal of Biochemistry & Cell Biology
Eichi TsurugaKazuharu Irie

Abstract

Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited. Microfibril-associated glycoprotein-1 (MAGP-1) and fibrillin-2, the major components of microfibrils, provide the likely template for tropoelastin deposition. In this study, we used the RNA interference (RNAi) technique to establish MAGP-1 and fibrillin-2 gene-specific knock-downs individually in elastin-producing cells (human gingival fibroblasts). We then examined the extracellular deposition of tropoelastin by western blotting. These two genes were specifically suppressed to < 30% of the control level, and this was responsible for the diminution of tropoelastin deposition. An immunofluorescence study also confirmed that RNAi-mediated down-regulation of MAGP-1 or fibrillin-2 led to the loss of tropoelastin immunoreactivity. These results suggest that MAGP-1 and fibrillin-2 are, directly or indirectly, associated with the extracellular deposition of tropoelastin during elastic fiber formation in human gingival fibroblasts in vitro.

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Dec 23, 2003·The International Journal of Biochemistry & Cell Biology·Eichi TsurugaKazuharu Irie

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Citations

Mar 31, 2006·Journal of Medical Genetics·P N RobinsonM Godfrey
Nov 18, 2009·Acta Histochemica Et Cytochemica·Yutaka HisanagaYoshihiko Sawa
Jan 20, 2011·Acta Histochemica Et Cytochemica·Yoshinori YamauchiHiroyuki Ishikawa
Aug 13, 2010·Connective Tissue Research·Yuka NakatomiHiroyuki Ishikawa
Nov 9, 2016·Frontiers in Physiology·Jiří Kanta
Mar 27, 2007·Journal of Dental Research·E TsurugaT Yajima

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