Microfluidic-aided genotyping of zebrafish in the first 48 h with 100% viability

Biomedical Microdevices
Raheel SamuelBruce K Gale

Abstract

This paper introduces an innovative method for genotyping 1-2 days old zebrafish embryos, without sacrificing the life/health of the embryos. The method utilizes microfluidic technology to extract and collect a small amount of genetic material from the chorionic fluid or fin tissue of the embryo. Then, using conventional DNA extraction, PCR amplification, and high resolution melt analysis with fluorescent DNA detection techniques, the embryo is genotyped. The chorionic fluid approach was successful 78% of the time while the fin clipping method was successful 100% of the time. Chorionic fluid was shown to only contain DNA from the embryo and not from the mother. These results suggest a novel method to genotype zebrafish embryos that can facilitate high-throughput screening, while maintaining 100% viability of the embryo.

References

Apr 19, 2007·Nature Reviews. Genetics·Graham J Lieschke, Peter D Currie
Oct 17, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kristen M KwanChi-Bin Chien
Nov 6, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·John M ParantH Joseph Yost
Apr 15, 2011·Lab on a Chip·Eric M WielhouwerMichael K Richardson
Dec 1, 1998·Analytical Chemistry·D C DuffyG M Whitesides

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Citations

Mar 1, 2016·Lab on a Chip·Fan YangZuanguang Chen
Dec 19, 2019·Zebrafish·Xue ZhangXin Lou
May 22, 2020·Frontiers in Molecular Biosciences·T Zhang, R T Peterson

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