Microfluidic and Nanofluidic Intracellular Delivery.

Advanced Science
Jeongsoo Hur, Aram J Chung

Abstract

Innate cell function can be artificially engineered and reprogrammed by introducing biomolecules, such as DNAs, RNAs, plasmid DNAs, proteins, or nanomaterials, into the cytosol or nucleus. This process of delivering exogenous cargos into living cells is referred to as intracellular delivery. For instance, clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 gene editing begins with internalizing Cas9 protein and guide RNA into cells, and chimeric antigen receptor-T (CAR-T) cells are prepared by delivering CAR genes into T lymphocytes for cancer immunotherapies. To deliver external biomolecules into cells, tools, including viral vectors, and electroporation have been traditionally used; however, they are suboptimal for achieving high levels of intracellular delivery while preserving cell viability, phenotype, and function. Notably, as emerging solutions, microfluidic and nanofluidic approaches have shown remarkable potential for addressing this open challenge. This review provides an overview of recent advances in microfluidic and nanofluidic intracellular delivery strategies and discusses new opportunities and challenges for clinical applications. Furthermore, key considerations for future efforts to develop ...Continue Reading

References

May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R Breslow, D L Huang
Aug 1, 1991·Biophysical Journal·T Y Tsong
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M FechheimerS G Zimmer
Jul 11, 1988·Nucleic Acids Research·W J DowerC W Ragsdale
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M FrommV Walbot
May 1, 1971·Biotechnology and Bioengineering·D C AugensteinD I Wang
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J W GordonF H Ruddle
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H PotterP Leder
Jul 10, 1996·Human Gene Therapy·H J KimM E Bolander
Jan 1, 1997·Ultrasound in Medicine & Biology·S BaoD L Miller
May 8, 1998·Analytical Biochemistry·A S Waldman, B C Waldman
Jul 4, 1998·Ultrasound in Medicine & Biology·W J GreenleafJ F Greenleaf
May 6, 1999·Bioelectrochemistry and Bioenergetics·R Stapulionis
Jan 29, 2000·Biotechnology and Bioengineering·D V SchafferD A Lauffenburger
Jul 11, 2000·Nature Biotechnology·T Vo-DinhG D Griffin
Sep 13, 2000·Antisense & Nucleic Acid Drug Development·C Ghosh, P L Iversen
May 29, 2002·Tissue Engineering·Astrid HammAnja K Bosserhoff
May 3, 2003·Nature Reviews. Genetics·Clare E ThomasMark A Kay
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Paul L McNeil, Richard A Steinhardt
Apr 22, 2004·Lab on a Chip·Sanghoon LeeDavid J Beebe
Dec 24, 2004·Lab on a Chip·Michelle KhineLuke P Lee
Mar 11, 2005·Advanced Drug Delivery Reviews·Sophie Mehier-Humbert, Richard H Guy
Jul 18, 2006·Analytical Chemistry·Hsiang-Yu Wang, Chang Lu
Sep 5, 2006·Biophysical Journal·Claus-Dieter OhlDetlef Lohse
May 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xing ChenCarolyn R Bertozzi
May 23, 2007·Journal of the American Chemical Society·Woong KimPeidong Yang
Jun 30, 2007·Analytical Chemistry·Zhengzheng FeiL James Lee
Jul 4, 2007·Nature Reviews. Genetics·Reinhard WaehlerDavid T Curiel
Jul 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·François O LaforgeMichael V Mirkin
Jul 21, 2007·Nature Protocols·Manuel ZeitelhoferRalf Dahm
Aug 7, 2007·Physical Review Letters·Ed ZwaanClaus-Dieter Ohl
Sep 11, 2007·Biomedical Microdevices·Cristian Ionescu-ZanettiMichelle Khine
Sep 20, 2007·Biotechnology and Bioengineering·Daniel M HallowMark R Prausnitz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.

Related Papers

Chemical Society Reviews
A van den BergPeidong Yang
Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Jongyoon Han, Jianping Fu
Microsystems & Nanoengineering
Oliver VanderpoortenTuomas P J Knowles
© 2021 Meta ULC. All rights reserved