Microfluidic auto-alignment of protein patterns for dissecting multi-receptor crosstalk in platelets

Lab on a Chip
F ZhouH Lu

Abstract

Cell adhesion plays a critical role in many cellular functions, such as the hemostatic/thrombotic process, inflammatory reactions, and adaptive immune response. Many cell adhesion processes involve crosstalk between multiple ligand-receptor systems through intracellular signaling. To elucidate such crosstalk requires analysis of the synergistic or antagonistic effects of binding and signalling of multi-receptor species. Current techniques for these analyses, e.g., atomic force microscopy (AFM) and biomembrane force probe (BFP) assays, are either labor-intensive, low-throughput, or limited in the types of ligands they can interrogate. Circumventing these limitations requires a technique for manipulating ligand interactions with and measuring the functional response of a population of cells. In this work, we have developed a microfluidic platform for studying the binding and signaling of multi-receptor species by separating their actions in space and time. The platform directs cells through a single channel and uses sequentially presented ligands for pre-processing and stimulating cells, followed by reporting of cell activation states and functional consequences. Our method precisely patterns multiple proteins in different spatia...Continue Reading

References

May 1, 1994·Thrombosis Research·S DittmarA R Gear
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·S TakayamaG M Whitesides
Dec 30, 1999·Biomaterials·R S KaneG M Whitesides
Jun 30, 2000·Biochemical and Biophysical Research Communications·V LeytinJ Freedman
Apr 16, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Jasper A RemijnPaquita Nurden
Jan 11, 2003·The Journal of Biological Chemistry·William HanleyKonstantinos Konstantopoulos
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jens M ChemnitzJames L Riley
Oct 3, 2008·Blood Cells, Molecules & Diseases·Mohammed El Haouari, Juan A Rosado
May 26, 2009·Nature Medicine·Warwick S NesbittShaun P Jackson
Apr 28, 2011·Lab on a Chip·Erwin BerthierDavid J Beebe
Sep 16, 2011·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·F SantilliG Davì
Nov 9, 2011·Nature Medicine·Shaun P Jackson
Oct 31, 2014·Nature Reviews. Molecular Cell Biology·Jay D HumphreyMartin A Schwartz
Sep 29, 2017·Molecular Biology of the Cell·Yunfeng ChenCheng Zhu

❮ Previous
Next ❯

Methods Mentioned

BETA
chip

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.