Microfluidic-Based Cell-Embedded Microgels Using Nonfluorinated Oil as a Model for the Gastrointestinal Niche

ACS Applied Materials & Interfaces
Seyed Ramin PajoumshariatiAlireza Abbaspourrad

Abstract

Microfluidic-based cell encapsulation has promising potential in therapeutic applications. It also provides a unique approach for studying cellular dynamics and interactions, though this concept has not yet been fully explored. No in vitro model currently exists that allows us to study the interaction between crypt cells and Peyer's patch immune cells because of the difficulty in recreating, with sufficient control, the two different microenvironments in the intestine in which these cell types belong. However, we demonstrate that a microfluidic technique is able to provide such precise control and that these cells can proliferate inside microgels. Current microfluidic-based cell microencapsulation techniques primarily use fluorinated oils. Herein, we study the feasibility and biocompatibility of different nonfluorinated oils for application in gastrointestinal cell encapsulation and further introduce a model for studying intercellular chemical interactions with this approach. Our results demonstrate that cell viability is more affected by the solidification and purification processes that occur after droplet formation rather than the oil type used for the carrier phase. Specifically, a shorter polymer cross-linking time and con...Continue Reading

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Citations

Jan 19, 2020·Biophysics Reviews·Seyedeh Sarah SalehiSiamak Kazemzadeh Hannani
Mar 14, 2020·International Journal of Molecular Sciences·Lixia HuangGuang Yang
Aug 1, 2019·Advanced Functional Materials·Xia CaoYu Shrike Zhang
Nov 14, 2020·Advanced Healthcare Materials·Sung-Min KangJoong Yull Park
Feb 9, 2021·Biosensors & Bioelectronics·Morteza AziziAlireza Abbaspourrad
May 2, 2019·ACS Applied Materials & Interfaces·Farrokh SharifiNicole N Hashemi
Nov 27, 2018·Analytical Chemistry·Morteza AziziAlireza Abbaspourrad
Nov 27, 2019·Analytical Chemistry·Yun DingAndrew J deMello
Jan 8, 2019·Journal of Agricultural and Food Chemistry·Morteza AziziAlireza Abbaspourrad

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