Microfluidic-Based Single-Cell Study: Current Status and Future Perspective

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Haiwa WuJingyao Sun

Abstract

Investigation of cell behavior under different environments and manual operations can give information in specific cellular processes. Among all cell-based analysis, single-cell study occupies a peculiar position, while it can avoid the interaction effect within cell groups and provide more precise information. Microfluidic devices have played an increasingly important role in the field of single-cell study owing to their advantages: high efficiency, easy operation, and low cost. In this review, the applications of polymer-based microfluidics on cell manipulation, cell treatment, and cell analysis at single-cell level are detailed summarized. Moreover, three mainly types of manufacturing methods, i.e., replication, photodefining, and soft lithography methods for polymer-based microfluidics are also discussed.

References

Jul 15, 1997·Analytical Chemistry·R M McCormickH H Hooper
Nov 2, 2004·Analytical Chemistry·Palaniappan SethuMehmet Toner
Dec 15, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Jessamine Ng LeeGeorge M Whitesides
Dec 24, 2004·Lab on a Chip·Anna TourovskaiaAlbert Folch
Jan 27, 2005·Lab on a Chip·Dino Di CarloLuke P Lee
Oct 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xiaoyuan HuHyongsok T Soh
Mar 3, 2006·Lab on a Chip·Daniel Irimia, Mehmet Toner
Jun 15, 2006·Analytical and Bioanalytical Chemistry·Chantal G Khan Malek
Jun 15, 2006·Analytical and Bioanalytical Chemistry·Chantal G Khan Malek
Jul 28, 2006·Nature·George M Whitesides
Oct 27, 2006·Lab on a Chip·Dino Di CarloLuke P Lee
Dec 26, 2006·Analytical Chemistry·Dino Di Carlo, Luke P Lee
Feb 12, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei-Hua HuangJie-Ke Cheng
Jul 24, 2008·Accounts of Chemical Research·Qiaobing XuGeorge M Whitesides
Oct 31, 2008·Talanta·Holger Becker, Laurie E Locascio
Jul 29, 2009·Lab on a Chip·William LiuEdward J Park
Mar 6, 2010·Nature Protocols·Dong QinGeorge M Whitesides
Nov 13, 2010·Small·Leslie Y YeoJames R Friend
Jan 29, 2011·Lab on a Chip·Soojung Claire HurDino Di Carlo
Aug 2, 2011·Lab on a Chip·Albert J MachDino Di Carlo
Aug 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Adam K WhiteCarl L Hansen
Aug 25, 2011·Lab on a Chip·Kazunori HoshinoXiaojing Zhang
Oct 18, 2011·Nature Nanotechnology·Pouyan E BoukanyL James Lee
Jan 17, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Lucia ZemaAndrea Gazzaniga
Mar 1, 2012·Sensors·Chun-Ping JenNikolay A Maslov
Apr 7, 2012·Nature Protocols·Veronica Sanchez-FreireJoseph C Wu

❮ Previous
Next ❯

Citations

Oct 31, 2019·Dose-response : a Publication of International Hormesis Society·Xiaoxiang HeDaming Wu
Mar 5, 2020·Molecular Biology Reports·Carmela Rita BalistreriAlexander Vaiserman
May 1, 2021·Nanomaterials·Rui VitorinoVáclav Kašička
Aug 27, 2021·Analytical and Bioanalytical Chemistry·Shuting XuHuwei Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
optical tweezers
biosensor
transfection
flow cytometry
chip
biopsy
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved