Abstract
With advances in nanotechnology, particles with various size, shape, surface chemistry, and composition can be easily produced. Nano- and microparticles have been extensively explored in many industrial and clinical applications. Ensuring that the particles themselves are not possessing toxic effects to the biological system is of paramount importance. This paper describes a proof of concept method, in which a microfluidic system is used in conjunction with a cell microarray technique aiming to streamline the analysis of particle-cell interaction in a high throughput manner. Polymeric microparticles, with different particle surface functionalities, were first used to investigate the efficiency of particle-cell adhesion under dynamic flow. Silver nanoparticles (AgNPs, 10 nm in diameter) perfused at different concentrations (0 to 20 μg/mL) in parallel streams over the cell microarray exhibited a higher toxicity compared to the static culture in the 96-well-plate format. This developed microfluidic system can be easily scaled up to accommodate a larger number of microchannels for high throughput analysis of the potential toxicity of a wide range of particles in a single experiment.
References
Jul 1, 1993·The Laryngoscope·S Collins
Dec 22, 1999·Analytical Chemistry·A E KamholzP Yager
Apr 8, 2003·Combinatorial Chemistry & High Throughput Screening·T J BurkeK M Ervin
Jan 2, 2004·Cell Biology and Toxicology·A TiwariA M Seifalian
Oct 2, 2004·Cell Death and Differentiation·N SelznerP-A Clavien
Oct 26, 2005·Drug Discovery Today·Kewal K Jain
Feb 4, 2006·Science·Andre NelNing Li
Jan 30, 2008·Cardiovascular and Interventional Radiology·Joachim KettenbachThomas Rand
Jun 7, 2008·Biomacromolecules·Francesca CavalieriGaio Paradossi
Jun 24, 2008·Techniques in Vascular and Interventional Radiology·A Laurent
Feb 12, 2009·ACS Nano·Edward JanNicholas A Kotov
Apr 23, 2009·Advanced Drug Delivery Reviews·Clinton F Jones, David W Grainger
Oct 20, 2010·Biomicrofluidics·Sanjeev Kumar MahtoSeog Woo Rhee
Jan 12, 2011·Environmental Science & Technology·Bernd NowackMurray Height
Apr 26, 2011·Nature Nanotechnology·Eun Chul ChoYounan Xia
Jun 21, 2011·Lab on a Chip·Lukas RichterPeter Ertl
Oct 29, 2011·Analytical Chemistry·Donghyuk KimChristy L Haynes
Nov 22, 2011·Nanotechnology·Cristiana S O PauloLino S Ferreira
Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rachit AgarwalKrishnendu Roy
Mar 13, 2014·Small·Giuseppe VecchioNicolas H Voelcker
Oct 9, 2014·Angewandte Chemie·Tianmeng SunYounan Xia
Dec 5, 2014·Reports on Progress in Physics·Brian D PlouffeLaura H Lewis
May 8, 2015·PloS One·Ruth Wang'onduGeorge Miller
Oct 2, 2015·Beilstein Journal of Nanotechnology·Marina E VanceMatthew S Hull
Feb 24, 2016·Blood·Nicholas HubbardTroy R Torgerson
May 22, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Cláudia SaraivaLiliana Bernardino
Oct 7, 2016·Bioconjugate Chemistry·Malou Henriksen-LaceyLuis M Liz-Marzán
Jan 12, 2017·Lab on a Chip·Ziqiu TongNicolas H Voelcker
Citations
Jan 2, 2020·Biomicrofluidics·Scott McCormickCraig Priest
Dec 16, 2020·Biomacromolecules·E Eduardo AntunezMarkus Müllner
Aug 23, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Jiwei CuiFrank Caruso
Jul 31, 2018·ACS Applied Materials & Interfaces·Jinming LiJuan Pablo Giraldo
Sep 7, 2021·Lab on a Chip·Ziqiu TongJohn M Haynes