Microfluidic-chip-based multiple-locus variable-number tandem-repeat fingerprinting with new primer sets for methicillin-resistant Staphylococcus aureus.

Journal of Clinical Microbiology
Artur J SabatJan Maarten van Dijl

Abstract

The detection of outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) infections and a rapid and accurate identification of sources and routes of transmission should be conducted in hospital settings as early and swiftly as possible. In this study, we investigated the application potential of a new approach based on multiple-locus variable-number tandem-repeat fingerprinting (MLVF) and microfluidics technology for a rapid discrimination of MRSA lineages in outbreak settings. A total of 206 nonrepetitive MRSA isolates recovered from infected patients at the University Medical Center Groningen between 2000 and 2010 were tested. The results obtained by MLVF using microcapillary electrophoresis with newly designed primers were compared to those obtained by spa typing and multiple-locus variable-number tandem-repeat analysis (MLVA). The discriminatory power was 0.980 (107 patterns), 0.969 (85 allelic profiles), and 0.959 (66 types) for MLVF, MLVA, and spa typing, respectively. All methods tested showed a good concordance of results calculated by the adjusted Rand's coefficient method. Comparisons of data obtained by the three approaches allowed us to propose an 88% cutoff value for the similarity between any two MLVF patt...Continue Reading

References

Jun 5, 1998·Microbiology·Richard Frothingham, Winifred A Meeker-O'Connell
Aug 30, 2001·Journal of Clinical Microbiology·A M KlevytskaP Keim
Feb 4, 2006·Journal of Clinical Microbiology·M Aires-de-SousaW Witte
Oct 6, 2006·Journal of Clinical Microbiology·Artur SabatWaleria Hryniewicz
Sep 3, 2008·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·A W FriedrichM G R Hendrix
May 1, 2009·Emerging Infectious Diseases·Ruud H DeurenbergEllen E Stobberingh
Jan 20, 2010·PLoS Medicine·Hajo GrundmannUNKNOWN European Staphylococcal Reference Laboratory Working Group

❮ Previous
Next ❯

Citations

Jan 22, 2014·International Journal of Medical Microbiology : IJMM·Magdalena M van der Kooi-PolJan Maarten van Dijl
Apr 24, 2013·Critical Reviews in Biotechnology·Minhaz Uddin AhmedApril S Brown
Jun 22, 2013·International Journal of Medical Microbiology : IJMM·Monika A ChlebowiczGirbe Buist
Dec 23, 2014·Journal of Chromatography. a·Eric R Castro, Andreas Manz
Feb 7, 2015·PLoS Neglected Tropical Diseases·Nana Ama AmissahYmkje Stienstra
Sep 12, 2015·PLoS Neglected Tropical Diseases·Nana Ama AmissahYmkje Stienstra
Jun 16, 2016·Journal of Microbiological Methods·Monica ImperiRoberta Creti
Feb 7, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C GlasnerJ M van Dijl

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.