Microfluidic device architecture for electrochemical patterning and detection of multiple DNA sequences

Langmuir : the ACS Journal of Surfaces and Colloids
Elizabeth PavlovicH T Soh

Abstract

Electrochemical biosensors pose an attractive solution for point-of-care diagnostics because they require minimal instrumentation and they are scalable and readily integrated with microelectronics. The integration of electrochemical biosensors with microscale devices has, however, proven to be challenging due to significant incompatibilities among biomolecular stability, operation conditions of electrochemical sensors, and microfabrication techniques. Toward a solution to this problem, we have demonstrated here an electrochemical array architecture that supports the following processes in situ, within a self-enclosed microfluidic device: (a) electrode cleaning and preparation, (b) electrochemical addressing, patterning, and immobilization of sensing biomolecules at selected sensor pixels, (c) sequence-specific electrochemical detection from multiple pixels, and (d) regeneration of the sensing pixels. The architecture we have developed is general, and it should be applicable to a wide range of biosensing schemes that utilize gold-thiol self-assembled monolayer chemistry. As a proof-of-principle, we demonstrate the detection and differentiation of polymerase chain reaction (PCR) amplicons diagnostic of human (H1N1) and avian (H5N...Continue Reading

References

Apr 19, 2003·Journal of Colloid and Interface Science·Elisabeth PavlovicSven Oscarsson
Jul 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chunhai FanAlan J Heeger
Sep 5, 2003·Nucleic Acids Research·Youdong MaoQi Ouyang
Sep 2, 2004·Journal of the American Chemical Society·Chad E ImmoosMark W Grinstaff
Jul 26, 2005·Angewandte Chemie·Kazuhiro UemuraHiroki Oshio
Dec 7, 2005·Biosensors & Bioelectronics·Joseph Wang
Dec 22, 2005·Journal of the American Chemical Society·Yi XiaoAlan J Heeger
Jan 5, 2006·Journal of the American Chemical Society·Abd-Elgawad RadiCiara K O'Sullivan
Feb 8, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Rebecca Y LaiAlan J Heeger
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rebecca Y LaiAlan J Heeger
Jul 27, 2006·Expert Review of Medical Devices·David Huckle
Oct 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yi XiaoAlan J Heeger
Feb 1, 2007·Journal of the American Chemical Society·Xiaolei ZuoChunhai Fan
May 10, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Francesco RicciJames J Sumner

❮ Previous
Next ❯

Citations

Jun 16, 2010·Analytical and Bioanalytical Chemistry·Iuliana OitaDebby Mangelings
Sep 5, 2013·Analytical Chemistry·Michael D M DrydenAaron R Wheeler
Mar 6, 2010·Accounts of Chemical Research·Arica A Lubin, Kevin W Plaxco
Mar 1, 2012·Chemical Reviews·Emil Paleček, Martin Bartošík
May 13, 2011·Journal of the American Chemical Society·B Scott FergusonH Tom Soh
May 14, 2009·Chemical Communications : Chem Comm·Weiwei YangRebecca Y Lai
Nov 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jonathan D AdamsH Tom Soh
Nov 29, 2013·Science Translational Medicine·Brian Scott FergusonHyongsok Tom Soh
Dec 14, 2011·Sensors·Sabo Wada Dutse, Nor Azah Yusof
May 28, 2011·Colloids and Surfaces. B, Biointerfaces·Yong-Ho ChungJeong-Woo Choi
May 4, 2011·Biosensors & Bioelectronics·Dujuan LiHuaguang Lu
Nov 27, 2009·Biosensors & Bioelectronics·E KomarovaA Bogomolova
Mar 7, 2009·Biosensors & Bioelectronics·Christopher Batchelor-McAuleyRichard G Compton
Oct 16, 2015·Talanta·Stanislav L KarstenHiroyuki Fujita
Oct 6, 2009·Electrophoresis·Mònica MirJosep Samitier
Nov 28, 2008·Angewandte Chemie·Johann ElbazItamar Willner
Jan 13, 2011·Angewandte Chemie·Hyun Ok HamPhillip B Messersmith
Sep 15, 2009·Biosensors & Bioelectronics·Xiaoteng Luo, I-Ming Hsing
Apr 2, 2014·Biosensors & Bioelectronics·Anna MiodekHafsa Korri-Youssoufi
Mar 13, 2014·Biosensors & Bioelectronics·Syed Rahin AhmedEnoch Y Park
Aug 30, 2011·Journal of Virological Methods·Ronghui WangYanbin Li
Jul 10, 2009·Analytical Chemistry·Brian S FergusonH Tom Soh
Feb 26, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Joseph P Labukas, Gregory S Ferguson
Nov 17, 2017·Analytical Chemistry·Hamid R Lotfi Zadeh Zhad, Rebecca Y Lai
Mar 19, 2015·Accounts of Chemical Research·Kuangwen HsiehH Tom Soh

❮ Previous
Next ❯

Related Concepts