Microfluidic device to study arterial shear-mediated platelet-surface interactions in whole blood: reduced sample volumes and well-characterised protein surfaces

Biomedical Microdevices
Nigel KentAntonio J Ricco

Abstract

We report a novel device to analyze cell-surface interactions under controlled fluid-shear conditions on well-characterised protein surfaces. Its performance is demonstrated by studying platelets interacting with immobilised von Willebrand Factor at arterial vascular shear rates using just 200 μL of whole human blood per assay. The device's parallel-plate flow chamber, with 0.1 mm² cross sectional area and height-to-width ratio of 1:40, provides uniform, well-defined shear rates along the chip surface with negligible vertical wall effects on the fluid flow profile while minimizing sample volumetric flow. A coating process was demonstrated by ellipsometry, atomic force microscopy, and fluorescent immunostaining to provide reproducible, homogeneous, uniform protein layers over the 0.7 cm² cell-surface interaction area. Customized image processing quantifies dynamic cellular surface coverage vs. time throughout the whole-blood-flow assay for a given drug treatment or disease state. This device can track the dose response of anti-platelet drugs, is suitable for point-of-care diagnostics, and is designed for adaptation to mass manufacture.

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Citations

Oct 29, 2011·Analytical Chemistry·Donghyuk KimChristy L Haynes
Feb 26, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Bincy JoseLourdes Basabe-Desmonts
Jul 17, 2015·Scientific Reports·Jonathan CowmanDermot Kenny
Feb 24, 2011·IEEE Transactions on Bio-medical Engineering·Nigel J KentAntonio J Ricco
Oct 27, 2011·The Analyst·María José Santos-MartínezMarek Witold Radomski
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Feb 23, 2019·Biomedical Microdevices·Marisel De Jesús VegaCarol Barry
Jul 12, 2020·Bulletin of Experimental Biology and Medicine·Yu N AvtaevaZ A Gabbasov
Jul 26, 2019·Analytical Chemistry·Crispin SzydzikWarwick S Nesbitt

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