Abstract
We report a novel device to analyze cell-surface interactions under controlled fluid-shear conditions on well-characterised protein surfaces. Its performance is demonstrated by studying platelets interacting with immobilised von Willebrand Factor at arterial vascular shear rates using just 200 μL of whole human blood per assay. The device's parallel-plate flow chamber, with 0.1 mm² cross sectional area and height-to-width ratio of 1:40, provides uniform, well-defined shear rates along the chip surface with negligible vertical wall effects on the fluid flow profile while minimizing sample volumetric flow. A coating process was demonstrated by ellipsometry, atomic force microscopy, and fluorescent immunostaining to provide reproducible, homogeneous, uniform protein layers over the 0.7 cm² cell-surface interaction area. Customized image processing quantifies dynamic cellular surface coverage vs. time throughout the whole-blood-flow assay for a given drug treatment or disease state. This device can track the dose response of anti-platelet drugs, is suitable for point-of-care diagnostics, and is designed for adaptation to mass manufacture.
References
Jul 24, 1998·Biomaterials·H Elwing
Jul 24, 1998·Biomaterials·P TengvallB Liedberg
Aug 5, 1998·Biomaterials·P TengvallI Lundström
Sep 16, 1998·Circulation·S H TamM T Nakada
Jul 12, 2001·Annual Review of Biomedical Engineering·G M WhitesidesD E Ingber
Oct 3, 2001·BMC Immunology·D C Brown, R S Larson
Feb 13, 2002·Analytical Chemistry·Travis D BooneStephen J Williams
Sep 15, 2004·Analytical Chemistry·Hang LuKlavs F Jensen
Nov 10, 2004·Biophysical Journal·Nipa A ModyMichael R King
Dec 18, 2004·Blood Reviews·Paul Harrison
Dec 24, 2004·Lab on a Chip·Wesley C ChangDorian Liepmann
Oct 20, 2005·Journal of Bioscience and Bioengineering·K NakanishiK Imamura
Mar 8, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Alicia Toscano, Maria M Santore
Jul 28, 2006·Nature·Jamil El-AliKlavs F Jensen
Aug 30, 2006·Journal of Thrombosis and Haemostasis : JTH·J J ZwagingaUNKNOWN Biorheology Subcommittee of the SSC of the ISTH
Aug 30, 2006·Journal of Thrombosis and Haemostasis : JTH·J J ZwagingaUNKNOWN Biorheology Subcommittee of the SSC of the Isth
Mar 29, 2007·Lab on a Chip·Ulrich Y SchaffScott I Simon
Oct 20, 2007·Journal of Thrombosis and Haemostasis : JTH·J J ZwagingaJ W M Heemskerk
Jan 26, 2008·Analytical Chemistry·Brian J SchmidtMichael B Lawrence
Mar 25, 2008·Annual Review of Biomedical Engineering·Paul YagerJohn Gerdes
Jul 24, 2008·Annual Review of Biomedical Engineering·Wendy Thomas
Sep 27, 2008·Lab on a Chip·Edgar GutierrezAna Kasirer-Friede
Oct 31, 2008·Talanta·Holger Becker, Laurie E Locascio
Nov 22, 2008·Lab on a Chip·Ivan K DimovLuke P Lee
Sep 22, 2009·Biomaterials·Nima SaeidiJeffrey W Ruberti
Dec 8, 2009·Lab on a Chip·Christian FillaferMatthias F Schneider
Jun 2, 2010·Analytical Biochemistry·Bryan LincolnGerardene Meade
Citations
Mar 29, 2013·Analytical and Bioanalytical Chemistry·Margaret B LucittDermot Kenny
Feb 15, 2012·Biomedical Microdevices·Piero MiloroPaolo Dario
Oct 29, 2011·Analytical Chemistry·Donghyuk KimChristy L Haynes
Feb 26, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Bincy JoseLourdes Basabe-Desmonts
Jul 17, 2015·Scientific Reports·Jonathan CowmanDermot Kenny
Oct 25, 2011·IEEE Transactions on Bio-medical Engineering·Sinead O'BrienGerardene Meade
Feb 24, 2011·IEEE Transactions on Bio-medical Engineering·Nigel J KentAntonio J Ricco
Feb 5, 2014·Redox Biology·T G WalshP Metharom
Oct 28, 2016·Journal of Thrombosis and Haemostasis : JTH·J CowmanD Kenny
Sep 27, 2018·Platelets·Jonathan CowmanEimear Dunne
Jan 16, 2019·Blood·Eimear DunneDermot Kenny
Oct 27, 2011·The Analyst·María José Santos-MartínezMarek Witold Radomski
Jul 28, 2019·Current Opinion in Hematology·Emilie MontenontRobert A Campbell
Dec 5, 2013·The Analyst·Donghyuk KimChristy L Haynes
Apr 23, 2014·Journal of Biomedical Materials Research. Part a·Megan A JamiolkowskiWilliam R Wagner
Oct 16, 2016·Cardiovascular Engineering and Technology·Adam RalphSimon Wong
Jul 27, 2017·Scientific Reports·Jonathan CowmanDermot Kenny
Aug 9, 2018·Clinical Kidney Journal·Claire KennedyDermot Kenny
Aug 1, 2018·Frontiers in Bioengineering and Biotechnology·Marbod WeberMeltem Avci-Adali
Feb 23, 2019·Biomedical Microdevices·Marisel De Jesús VegaCarol Barry
Jul 12, 2020·Bulletin of Experimental Biology and Medicine·Yu N AvtaevaZ A Gabbasov
Dec 13, 2017·Polymers·Joanna WardBrian J Meenan
Oct 13, 2020·Biomaterials Science·Jun Ki HongAnna Waterhouse
May 17, 2019·ACS Sensors·Weiwei LiJin-Ming Lin
Jul 26, 2019·Analytical Chemistry·Crispin SzydzikWarwick S Nesbitt