Microfluidic devices for the study of actin cytoskeleton in constricted environments: Evidence for podosome formation in endothelial cells exposed to a confined slit

Methods : a Companion to Methods in Enzymology
Pirjo SpuulElisabeth Génot

Abstract

The study of cell behavior in constricted environment is particularly relevant to our understanding of the mechanisms of cell invasion. In this regard, microfluidic systems offer promising platforms as microfabricated fluidic chips provide well-controlled physical, chemical and confined environments to study cell phenotype and behavior. Here, we report a fast and effective manufacturing process of user-friendly microfluidic chips ideally suited for quantitative live cell analysis in combination with immunofluorescence microscopy. The chip body, made of polydimethylsiloxane, is composed of two incubation chambers connected by one rectangular intermediate entry channel which provides access to a series of transversal slits where the observation can be made. The height of the slit is designed to be slightly smaller than that of the cells under study. To validate the chip performance, we analyzed the reorganization of the cytoskeleton of endothelial cells under various degree of spatial confinement. We illustrate how the constricted environment affects endothelial cell behavior in inducing the formation of podosomes. Moreover, the process was stimulated further when the surface of the slit was coated with a thin layer of fibronecti...Continue Reading

References

Jul 18, 2002·Accounts of Chemical Research·J Cooper McDonald, George M Whitesides
Nov 13, 2003·Electrophoresis·Samuel K Sia, George M Whitesides
Dec 4, 2003·Analytical Chemistry·Jessamine Ng LeeGeorge M Whitesides
Dec 15, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Jessamine Ng LeeGeorge M Whitesides
Jun 10, 2008·Nature Methods·Julia RiedlRoland Wedlich-Soldner
Aug 15, 2009·Trends in Cardiovascular Medicine·Zorina S Galis, Susan M Lessner
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Daniel Irimia, Mehmet Toner
Nov 25, 2011·Molecular Biology of the Cell·Amélie JuinFrédéric Saltel
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Aug 28, 2013·The Journal of Cell Biology·Wei-Chien HungKonstantinos Konstantopoulos
Oct 15, 2013·Immunological Reviews·Mélina L HeuzéAna-Maria Lennon-Duménil
Oct 11, 2014·Cellular and Molecular Life Sciences : CMLS·Stefan Linder, Christiane Wiesner
Feb 14, 2015·Cell·Verena RuprechtCarl-Philipp Heisenberg

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Citations

Feb 21, 2016·Methods : a Companion to Methods in Enzymology·Nicolas BorghiChristophe Lavelle
May 28, 2016·Journal of Cell Science·Thomas DaubonElisabeth Génot
Oct 11, 2016·Nature Communications·Marjolein B M MeddensAlessandra Cambi
Feb 29, 2020·Current Opinion in Hematology·Florian AlonsoElisabeth Génot
Sep 3, 2020·International Journal of Molecular Sciences·Haibo HuangLining Sun
May 23, 2020·European Journal of Cell Biology·Florian AlonsoElisabeth Génot

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