Microfluidic Distance Readout Sweet Hydrogel Integrated Paper-Based Analytical Device (μDiSH-PAD) for Visual Quantitative Point-of-Care Testing

Analytical Chemistry
Xiaofeng WeiChaoyong James Yang

Abstract

A disposable, equipment-free, versatile point-of-care testing platform, microfluidic distance readout sweet hydrogel integrated paper-based analytical device (μDiSH-PAD), was developed for portable quantitative detection of different types of targets. The platform relies on a target-responsive aptamer cross-linked hydrogel for target recognition, cascade enzymatic reactions for signal amplification, and microfluidic paper-based analytic devices (μPADs) for visual distance-based quantitative readout. A "sweet" hydrogel with trapped glucoamylase (GA) was synthesized using an aptamer as a cross-linker. When target is present in the sample, the "sweet" hydrogel collapses and releases enzyme GA into the sample, generating glucose by amylolysis. A hydrophilic channel on the μPADs is modified with glucose oxidase (GOx) and colorless 3,3'-diaminobenzidine (DAB) as the substrate. When glucose travels along the channel by capillary action, it is converted to H2O2 by GOx. In addition, DAB is converted into brown insoluble poly-3,3'-diaminobenzidine [poly(DAB)] by horseradish peroxidase, producing a visible brown bar, whose length is positively correlated to the concentration of targets. The distance-based visual quantitative platform can ...Continue Reading

References

Sep 29, 2000·Pharmacology & Therapeutics·J Spychala
Jul 18, 2001·Journal of the American Chemical Society·M N StojanovicD W Landry
Jan 4, 2006·Journal of Applied Toxicology : JAT·P FernándezM J Tabernero
Jan 11, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Rosa Cordero, Sue Paterson
Jan 11, 2007·Angewandte Chemie·Andres W MartinezGeorge M Whitesides
Aug 25, 2007·Chemical Reviews·Michael FamulokGünter Mayer
May 13, 2011·Journal of the American Chemical Society·B Scott FergusonH Tom Soh
Mar 1, 2012·Chemical Reviews·Tina VermondenWim E Hennink
Aug 14, 2012·Analytical Chemistry·Debolina ChatterjeeAdam T Woolley
Oct 4, 2012·Chemical Society Reviews·Claudio Parolo, Arben Merkoçi
Dec 20, 2012·Nature Communications·Yujun SongLidong Qin
Jan 24, 2013·Journal of the American Chemical Society·Ling YanChaoyong James Yang
Feb 9, 2013·Analytical Chemistry·Rui-Qiao ZhangZhi-Ling Zhang
May 9, 2013·Lab on a Chip·Ali Kemal YetisenChristopher R Lowe
May 10, 2013·Lab on a Chip·David M CateCharles S Henry
Aug 13, 2013·Analytical Chemistry·Christophe RenaultRichard M Crooks
Oct 29, 2013·Journal of the American Chemical Society·Yujun SongLidong Qin
Jan 8, 2014·Biomicrofluidics·Suk-Heung SongSehyun Shin
Mar 1, 2014·Analytical Chemistry·Poomrat RattanaratCharles S Henry
Apr 22, 2014·Chemical Reviews·Onur TokelUtkan Demirci
Jun 3, 2014·Chemical Society Reviews·Jiashu SunXingyu Jiang
Jun 21, 2014·Chemical Communications : Chem Comm·Qiu-Mei FengHong-Yuan Chen
Jul 16, 2014·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Kiwing To
Sep 13, 2014·Angewandte Chemie·Kha TramYingfu Li
Oct 24, 2014·Analytical Chemistry·Yishun HuangChaoyong James Yang
Nov 29, 2014·Analytical Methods : Advancing Methods and Applications·C K TangJ F Rusling
Dec 23, 2014·Analytical Methods : Advancing Methods and Applications·Debolina ChatterjeeAdam T Woolley

❮ Previous
Next ❯

Citations

Jul 13, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Hua Tian, Junhui He
Dec 13, 2016·Analytical Chemistry·Yuanyuan YangCharles S Henry
May 10, 2017·Biotechnology Journal·Pia GruberNicolas Szita
Aug 2, 2018·Advanced Materials·Yan ZhangHong Liu
Jan 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yuhi Shimada, Takashi Kaneta
Jan 17, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rudi LiuYingfu Li
Sep 12, 2019·Scientific Reports·Tuchpongpuch BoonpoempoonTakashi Kaneta
Apr 4, 2017·Advanced Healthcare Materials·Il Young JungHyukjin Lee
Jan 28, 2020·Annual Review of Analytical Chemistry·Tugba OzerCharles S Henry
Aug 8, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Zhaoliang GuoGuifu Zuo
Dec 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yue ZhangYanfei Shen
May 1, 2019·Advanced Biosystems·Lewis A FraserJulian A Tanner
Jan 23, 2020·Biomicrofluidics·Md Almostasim MahmudBrendan D MacDonald
Nov 24, 2016·Science and Technology of Advanced Materials·Yasuhiko IwasakiRui Moriyama
Jul 28, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sangkaew PrapapornDungchai Wijitar
Oct 7, 2020·Biosensors & Bioelectronics·Tao MingXinxia Cai
Mar 20, 2021·Analytica Chimica Acta·Kawin KhachornsakkulChih-Hsin Chen
Jun 15, 2021·Chemical Reviews·Eka NovianaCharles S Henry
Jun 17, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Lidya AbuneYong Wang
Aug 14, 2021·Biosensors & Bioelectronics·Jiantao PingQiongzheng Hu
Mar 15, 2019·Analytical Chemistry·Chang ChenHuaying Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.