Microfluidic-Enabled Print-to-Screen Platform for High-Throughput Screening of Combinatorial Chemotherapy

Analytical Chemistry
Yuzhe DingTingrui Pan

Abstract

Since the 1960s, combination chemotherapy has been widely utilized as a standard method to treat cancer. However, because of the potentially enormous number of drug candidates and combinations, conventional identification methods of the effective drug combinations are usually associated with significantly high operational costs, low throughput screening, laborious and time-consuming procedures, and ethical concerns. In this paper, we present a low-cost, high-efficiency microfluidic print-to-screen (P2S) platform, which integrates combinatorial screening with biomolecular printing for high-throughput screening of anticancer drug combinations. This P2S platform provides several distinct advantages and features, including automatic combinatorial printing, high-throughput parallel drug screening, modular disposable cartridge, and biocompatibility, which can potentially speed up the entire discovery cycle of potent drug combinations. Microfluidic impact printing utilizing plug-and-play microfluidic cartridges is experimentally characterized with controllable droplet volume and accurate positioning. Furthermore, the combinatorial print-to-screen assay is demonstrated in a proof-of-concept biological experiment which can identify the ...Continue Reading

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Citations

May 24, 2016·Advanced Drug Delivery Reviews·Ruiwu LiuKit S Lam
Oct 23, 2016·Talanta·Hui DongJianping Zheng
Jun 5, 2018·Biomicrofluidics·Jinzhen FanTingrui Pan
Aug 24, 2017·Scientific Reports·Ada Hang-Heng WongChu-Xia Deng
Apr 30, 2019·Advanced Materials·Daniela S MattesFrank Breitling
Nov 27, 2019·Biomicrofluidics·Jiahui SunXianting Ding
Jan 7, 2021·Annals of Biomedical Engineering·Farbod AmirghasemiMehdi Nikkhah
Jan 29, 2020·Analytica Chimica Acta·Magdalena FlontZbigniew Brzózka
Aug 1, 2021·Drug Discovery Today·Boqian WangXianting Ding

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