Microfluidic fabrication of bioactive microgels for rapid formation and enhanced differentiation of stem cell spheroids

Acta Biomaterialia
Christian SiltanenAlexander Revzin

Abstract

A major challenge in tissue engineering is to develop robust protocols for differentiating ES and iPS cells to functional adult tissues at a clinically relevant scale. The goal of this study is to develop a high throughput platform for generating bioactive, stem cell-laden microgels to direct differentiation in a well-defined microenvironment. We describe a droplet microfluidics system for fabricating microgels composed of polyethylene glycol and heparin, with tunable geometric, mechanical, and chemical properties, at kHz rates. Heparin-containing hydrogel particles sequestered growth factors Nodal and FGF-2, which are implicated in specifying pluripotent cells to definitive endoderm. Mouse ESCs were encapsulated into heparin microgels with a single dose of Nodal and FGF-2, and expressed high levels of endoderm markers Sox17 and FoxA2 after 5 days. These results highlight the use of microencapsulation for tailoring the stem cell microenvironment to promote directed differentiation, and may provide a straightforward path to large scale bioprocessing in the future. Multicellular spheroids and microtissues are valuable for tissue engineering, but fabrication approaches typically sacrifice either precision or throughput. Microfluid...Continue Reading

References

May 17, 2006·Acta Biomaterialia·Danielle S W Benoit, Kristi S Anseth
Mar 28, 2007·Nature Reviews. Genetics·Patrick P L Tam, David A F Loebel
May 7, 2008·Biomacromolecules·Won Il ChoiYoung Ha Kim
Jun 21, 2008·Biotechnology Journal·Ruei-Zeng LinHwan-You Chang
Feb 7, 2009·Biotechnology Progress·Andrés M Bratt-LealTodd C McDevitt
Sep 17, 2010·The Journal of Biological Chemistry·Shaoliang LiKiyotoshi Sekiguchi
Mar 9, 2011·Biochemical and Biophysical Research Communications·Mahboobe GhaediAlexander Revzin
Feb 10, 2012·Lab on a Chip·Mira T GuoDavid A Weitz
Feb 24, 2012·Journal of the American Chemical Society·Torsten RossowSebastian Seiffert
Aug 25, 2012·Results and Problems in Cell Differentiation·Peter T W Kim, Christopher J Ong
Dec 15, 2012·Biotechnology and Bioengineering·Jenna L Wilson, Todd C McDevitt
Oct 30, 2013·Biotechnology and Bioengineering·Jenna L WilsonTodd C McDevitt
Sep 4, 2014·Chemical Communications : Chem Comm·David G BelairWilliam L Murphy

❮ Previous
Next ❯

Citations

Mar 21, 2018·Regenerative Medicine·Juan Pablo AcevedoMaroun Khoury
Nov 1, 2016·Lab on a Chip·Weiqian JiangKam W Leong
Jul 24, 2018·Small·Garima Agrawal, Rahul Agrawal
Aug 1, 2020·Advanced Healthcare Materials·Se-Jeong KimHeungsoo Shin
Jun 30, 2016·Nanomedicine·Abhay Pandit, Dimitrios I Zeugolis
Apr 16, 2020·Advanced Biosystems·Jie WangYuanjin Zhao
Apr 10, 2019·Polymers·Goeun ChoeJae Young Lee
Apr 16, 2020·Advanced Biosystems·Kosuke InoHitoshi Shiku
Feb 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chao HeChangsheng Zhao
Mar 13, 2020·Biomicrofluidics·Mohamed G A MohamedKeekyoung Kim
Sep 9, 2017·Nature Communications·Sébastien SartCharles N Baroud
May 23, 2018·Scientific Reports·Christian A SiltanenAdam R Abate
Nov 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Justin L MadrigalEduardo A Silva
Mar 25, 2019·Biomedical Microdevices·Marissa E WechslerNicholas A Peppas
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Bruna Gregatti de CarvalhoLucimara Gaziola de la Torre
Jan 17, 2021·Scientific Reports·Hamed ShiehDariush Bastani
Dec 5, 2017·Materials Science & Engineering. R, Reports : a Review Journal·Jeroen LeijtenAli Khademhosseini
Apr 13, 2021·Advanced Functional Materials·Alexander S CaldwellKristi S Anseth
May 1, 2021·Polymers·María Puertas-BartoloméLuis García-Fernández
Sep 21, 2017·ACS Applied Materials & Interfaces·Pengfei XiaJingbo Yin
Feb 19, 2019·ACS Sensors·Ali RahimianAlexander Revzin
Oct 2, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Saahil ShethSilviya P Zustiak
Oct 23, 2021·ACS Biomaterials Science & Engineering·Kazuhiko IshiharaKyoko Fukazawa
Dec 11, 2021·Biomacromolecules·Bruna G CarvalhoLucimara G de la Torre

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.