Microfluidic fabrication of microcarriers with sequential delivery of VEGF and BMP-2 for bone regeneration.

Scientific Reports
Erfan DashtimoghadamLobat Tayebi

Abstract

Wound instability and poor functional vascularization in bone tissue engineering lead to lack of tissue integration and ultimate failure of engineered grafts. In order to harness the regenerative potential of growth factors and stimulate bone healing, present study aims to design multifunctional cell therapy microcarriers with the capability of sequential delivery of essential growth factors, bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor (VEGF). An on-chip double emulsion method was implemented to generate monodisperse VEGF encapsulated microcarriers. Bio-inspired poly(3,4-dihydroxyphenethylamine) (PDA) was then functionalized to the microcarriers surface for BMP-2 conjugation. The microcarriers were seeded with mesenchymal stem cells (MSCs) using a dynamic culture technique for cells expansion. Finally, the microcarriers were incorporated into an injectable alginate-RGD hydrogel laden with endothelial cells (ECs) for further analysis. The DNA and calcium content, as well as ALP activity of the construct were analyzed. The confocal fluorescent microscopy was employed to monitor the MSCs and tunneling structure of ECs. Eventually, the capability of developed microcarriers for bone tissue formation w...Continue Reading

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Citations

Mar 23, 2021·Acta Biomaterialia·Sheng Yin, Yi Cao
Jun 9, 2021·Journal of Experimental & Clinical Cancer Research : CR·Jun SunQian Liu
Oct 12, 2021·Frontiers in Molecular Biosciences·Safa A Damiati, Samar Damiati

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Methods Mentioned

BETA
chip
ELISA
Fluorescence
Assay
fluorescence microscopy
PCR
scanning electron microscopy

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