Microfluidic mazes to characterize T-cell exploration patterns following activation in vitro

Integrative Biology : Quantitative Biosciences From Nano to Macro
Namrata G JainDaniel Irimia

Abstract

The migration of T-cell subsets within peripheral tissues is characteristic of inflammation and immunoregulation. In general, the lymphocyte migratory response is assumed directional and guided by local gradients of chemoattractants and/or chemorepellents. However, little is known about how cells explore their tissue environment, and whether lymphocyte activation may influence speed and exploratory patterns of migration. To probe migration patterns by T-cells we designed a microfluidic maze device that replicates critical features of a tissue-like microenvironment. We quantified the migration patterns of unstimulated and mitogen-activated human T-cells at single cell resolution and found significant differences in exploration within microfluidic mazes. While unstimulated lymphocytes migrated in a directed manner, activated T-cells migrated through large areas of the mazes in an exploratory pattern in response to the chemoattractants RANTES (CCL5) and IP-10 (CXCL10). The analysis of migration enabled by the microfluidic devices help develop new methods for determining how human circulating T-cells function in vivo to seek out antigens in health and disease states.

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Citations

Nov 12, 2016·Lab on a Chip·Yan MaQun Fang
May 20, 2016·Journal of Leukocyte Biology·Daniel Irimia, Felix Ellett
Oct 28, 2016·Journal of Biomechanical Engineering·Samuel G Rayner, Ying Zheng
Feb 1, 2018·Scientific Reports·Leo BoneschanskerDaniel Irimia
Apr 13, 2019·Immunological Reviews·Tim Lämmermann, Wolfgang Kastenmüller
Nov 22, 2018·Frontiers in Immunology·Nidhi SinhaJurjen Tel
Jul 20, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Leo BoneschanskerDavid M Briscoe

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