Microfluidic particle zipper enables controlled loading of droplets with distinct particle types.

Lab on a Chip
Cyrille L Delley, Adam R Abate

Abstract

Current encapsulation approaches control the number of particles encapsulated per droplet, but not the particle types; consequently, they are unable to generate droplets containing combinations of distinct particle types, limiting the reactions that can be performed. We describe a microfluidic particle zipper that allows the number and types of particles encapsulated in every droplet to be controlled. The approach exploits self-ordering to generate repeating particle patterns that allow controlled encapsulation in droplets. We use the method to combine barcode particles with gel encapsulated cells to profile multiple disease relevant genomic loci with single cell sequencing. Particle zippers can operate in series to generate complex particle compositions in droplets.

References

Jul 25, 2008·Lab on a Chip·Jon F EddMehmet Toner
Sep 25, 2008·Lab on a Chip·C HoltzeD A Weitz
Aug 26, 2009·Lab on a Chip·Adam R AbateDavid A Weitz
Oct 23, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Yukiko TsudaShoji Takeuchi
Apr 6, 2013·Nature Protocols·Linas MazutisJohn A Heyman
Mar 31, 2015·Frontiers in Microbiology·Manu V Tamminen, Marko P J Virta
Aug 2, 2016·Nature Methods·Liyi XuPaul C Blainey
Dec 9, 2016·Nature Protocols·Rapolas ZilionisLinas Mazutis
Oct 27, 2018·Lab on a Chip·Iain C Clark, Adam R Abate

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Citations

May 27, 2021·Scientific Reports·Cyrille L Delley, Adam R Abate
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Minjun ChenGoran T Vladisavljević
Nov 11, 2020·Analytical Chemistry·Simon F BerlandaPetra S Dittrich

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Methods Mentioned

BETA
fluorescence microscopy
PCR
genotyping
chip
biopsy

Software Mentioned

Fasta
scipy
numpy
UMAP

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