Microfluidic perfusion modulates growth and motor neuron differentiation of stem cell aggregates.

The Analyst
Emily L Jackson-HolmesHang Lu

Abstract

Microfluidic technologies provide many advantages for studying differentiation of three-dimensional (3D) stem cell aggregates, including the ability to control the culture microenvironment, isolate individual aggregates for longitudinal tracking, and perform imaging-based assays. However, applying microfluidics to studying mechanisms of stem cell differentiation requires an understanding of how microfluidic culture conditions impact cell phenotypes. Conventional cell culture techniques cannot directly be applied to the microscale, as microscale culture varies from macroscale culture in multiple aspects. Therefore, the objective of this work was to explore key parameters in microfluidic culture of 3D stem cell aggregates and to understand how these parameters influence stem cell behavior and differentiation. These studies were done in the context of differentiation of embryonic stem cells (ESCs) to motor neurons (MNs). We assessed how media exchange frequency modulates the biochemical microenvironment, including availability of exogenous factors (e.g. nutrients, small molecule additives) and cell-secreted molecules, and thereby impacts differentiation. The results of these studies provide guidance on how key characteristics of 3...Continue Reading

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Citations

Dec 31, 2020·International Journal of Molecular Sciences·Jerran SantosBruce K Milthorpe
Mar 31, 2021·Scientific Reports·Eleonora De VitisAlessandro Polini

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

ZEN
COMSOL
MATLAB
GraphPad Prism

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