Microfluidic platform for assessing pancreatic islet functionality through dielectric spectroscopy

Biomicrofluidics
K HeilemanM Tabrizian

Abstract

Human pancreatic islets are seldom assessed for dynamic responses to external stimuli. Thus, the elucidation of human islet functionality would provide insights into the progression of diabetes mellitus, evaluation of preparations for clinical transplantation, as well as for the development of novel therapeutics. The objective of this study was to develop a microfluidic platform for in vitro islet culture, allowing the multi-parametric investigation of islet response to chemical and biochemical stimuli. This was accomplished through the fabrication and implementation of a microfluidic platform that allowed the perifusion of islet culture while integrating real-time monitoring using impedance spectroscopy, through microfabricated, interdigitated electrodes located along the microchamber arrays. Real-time impedance measurements provide important dielectric parameters, such as cell membrane capacitance and cytoplasmic conductivity, representing proliferation, differentiation, viability, and functionality. The perifusion of varying glucose concentrations and monitoring of the resulting impedance of pancreatic islets were performed as proof-of-concept validation of the lab-on-chip platform. This novel technique to elucidate the unde...Continue Reading

References

Jan 1, 1992·Pflügers Archiv : European journal of physiology·K D Gillis, S Misler
Feb 1, 1988·The Journal of Clinical Investigation·K S PolonskyE Van Cauter
Apr 15, 2000·Pancreas·S ParaskevasL Rosenberg
Jul 6, 2000·European Biophysics Journal : EBJ·I ErmolinaY Feldman
Dec 1, 2000·Physics in Medicine and Biology·K Asami, A Irimajiri
Oct 11, 2001·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·M S Benninger
Jan 15, 2002·The Journal of Clinical Endocrinology and Metabolism·Soon H SongPeter C Butler
Feb 12, 2002·Physics in Medicine and Biology·Eugen GheorghiuMihaela Gheorghiu
Aug 9, 2003·Advances in Experimental Medicine and Biology·Y Murat ElçinThomas Linn
Aug 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jonathan V RocheleauDavid W Piston
Jul 28, 2006·Biosensors & Bioelectronics·K Vengatajalabathy GobiNorio Miura
Oct 19, 2006·Biosensors & Bioelectronics·Md Aminur RahmanYoon-Bo Shim
Nov 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Delai ChenRustem F Ismagilov
Jan 24, 2009·Annals of the New York Academy of Sciences·Matthias BraunPatrik Rorsman
Feb 12, 2009·Lab on a Chip·Javeed Shaikh MohammedDavid T Eddington
Mar 20, 2010·Biomedical Microdevices·Adeola F AdewolaYong Wang
Jun 26, 2013·Biosensors & Bioelectronics·Khalil HeilemanMaryam Tabrizian
May 20, 2014·Colloids and Surfaces. B, Biointerfaces·Koji Asami

❮ Previous
Next ❯

Citations

Nov 5, 2016·Colloids and Surfaces. B, Biointerfaces·Khalil Leon HeilemanMaryam Tabrizian
Feb 14, 2017·Nano Convergence·Patrick T J HwangHo-Wook Jun
Nov 30, 2016·Biological Procedures Online·Nicholas B WhitticarCraig S Nunemaker
Jan 22, 2021·Diabetes·Aaron L GliebermanKevin Kit Parker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.