Microfluidic platform for probing cancer cells migration property under periodic mechanical confinement

Biomicrofluidics
Dongce MaDong Sun

Abstract

Cancer cell migration and invasion, which are involved in tumour metastasis, are hard to predict and control. Numerous studies have demonstrated that physical cues influence cancer cell migration and affect tumour metastasis. In this study, we proposed the use of a microchannel chip equipped with a number of vertical constrictions to produce periodic compression forces on cells passing through narrow channels. The chip with repeated vertical confinement was applied on adherent MHCC-97L liver cancer cells and suspended OCI-AML leukaemia cells to determine the migration ability of these cancer cells. Given the stimulation of the periodic mechanical confinement on-chip, the migration ability of cancer cells was promoted. Moreover, the migration speed increased as the stimulation was enhanced. Both AFM nanoindentation and optical stretching tests on cancer cells were performed to measure their mechanical property. After confinement stimulation, the cancer cells possessed higher deformability and lower stiffness than non-stimulating cells. The confinement stimulation altered the cell cytoskeleton, which governs the migration speed. This phenomenon was determined through gene expression analysis. The proposed on-chip cell migration a...Continue Reading

References

Jun 1, 2001·Nature Medicine·C BremerR Weissleder
Jun 5, 2003·Nature Reviews. Cancer·Gabriele Bergers, Laura E Benjamin
Feb 22, 2005·Nature Cell Biology·Simon WilkinsonChristopher J Marshall
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Jun 23, 2007·Biomaterials·Fu-Qiang NieTeruo Okano
Jul 13, 2007·Immunological Reviews·Andrew SmithNancy Hogg
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Daniel Irimia, Mehmet Toner
Aug 31, 2010·Cytoskeleton·Mutsuki AmanoKozo Kaibuchi
Mar 2, 2011·PloS One·Shalini Menon, Karen A Beningo
Oct 21, 2011·Lab on a Chip·Elisabetta PrimiceriGiuseppe Maruccio
Nov 29, 2011·Cell·Peter Friedl, Stephanie Alexander
May 2, 2012·The Journal of Cell Biology·Ryan J PetrieKenneth M Yamada
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amit Pathak, Sanjay Kumar
Jul 4, 2012·Nature Materials·Jing LiuBo Huang
Dec 4, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marie Morgan-FisherAtsuko Yoneda
Feb 28, 2013·Biophysical Journal·Stephanie S ChangYu-li Wang
Oct 24, 2014·Lab on a Chip·Mayuree ChanasakulniyomJonathan M Cooper
Feb 14, 2015·Cell·Matthew D Welch
Apr 12, 2015·Biophysical Journal·Hao YangDong Sun
Apr 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sam H AuMehmet Toner
Jul 16, 2016·Annual Review of Biomedical Engineering·Colin D PaulKonstantinos Konstantopoulos
Nov 7, 2016·Biosensors & Bioelectronics·Yaping LiMengsu Yang
Nov 12, 2016·Lab on a Chip·Yan MaQun Fang
Dec 3, 2016·Nature Reviews. Cancer·Colin D PaulKonstantinos Konstantopoulos
Aug 23, 2017·Scientific Reports·Xiaoqing LvHongda Chen

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Citations

Dec 27, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ning LvLi Yuan
Oct 23, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ke WangJian Chen
Nov 19, 2019·Biomicrofluidics·Zihui WangZuobin Wang
Jan 2, 2020·APL Bioengineering·Gregory H Underhill, Salman R Khetani
Jun 25, 2020·Seminars in Cancer Biology·Amanda M ClarkAlan Wells
Mar 2, 2021·Frontiers in Bioengineering and Biotechnology·Sebastian E Amos, Yu Suk Choi
Jun 18, 2021·Seminars in Liver Disease·David A Kukla, Salman R Khetani

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