Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples

Human Reproduction
Molly M QuinnMitchell P Rosen

Abstract

Does microfluidic sorting improve the selection of sperm with lower DNA fragmentation over standard density-gradient centrifugation? Microfluidic sorting of unprocessed semen allows for the selection of clinically usable, highly motile sperm with nearly undetectable levels of DNA fragmentation. Microfluidic devices have been explored to sort motile and morphologically normal sperm from a raw sample without centrifugation; however, it is uncertain whether DNA damage is reduced in this process. This is a blinded, controlled laboratory study of differences in standard semen analysis parameters and the DNA fragmentation index (DFI) in split samples from infertile men (n = 70) that were discarded after routine semen analysis at an academic medical center. Sperm concentration, progressive motility and forward progression were assessed by microscopic examination. For each sample, the unprocessed semen was tested for DNA fragmentation and split for processing by density-gradient centrifugation with swim-up or sorting by a microfluidic chip. DNA fragmentation was assessed in unprocessed and processed samples by sperm chromatin dispersion assay. The DFI was calculated, from up to 300 cells per slide, as the number of cells with fragmente...Continue Reading

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Citations

May 17, 2019·Lab on a Chip·Jason RiordonDavid Sinton
May 17, 2019·Journal of Assisted Reproduction and Genetics·Koray Yildiz, Sengul Yuksel
Aug 17, 2019·Journal of Assisted Reproduction and Genetics·Alessandra ParrellaGianpiero D Palermo
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May 31, 2019·F1000Research·Jonathan Fainberg, James A Kashanian
Jun 27, 2019·Micromachines·Haoqing ZhangPavel Neuzil
Dec 19, 2019·Frontiers in Cell and Developmental Biology·Iván Oseguera-LópezSerafín Pérez-Cerezales
Jan 17, 2021·Systems Biology in Reproductive Medicine·Fatemeh AnbariMojdeh Sabour
Jan 28, 2021·Human Fertility : Journal of the British Fertility Society·Jorge TenRafael Bernabeu
Nov 12, 2020·Advanced Science·Fernando SotoUtkan Demirci
Jan 22, 2021·The World Journal of Men's Health·Daniel GonzalezRanjith Ramasamy
Apr 11, 2020·The Urologic Clinics of North America·Brent M HansonJason M Franasiak
Mar 5, 2021·Journal of Assisted Reproduction and Genetics·Sandra Lara-CerrilloAgustín García-Peiró
Apr 4, 2021·Micromachines·Honeyeh Matbaechi Ettehad, Christian Wenger
Apr 4, 2021·Journal of Clinical Medicine·Caroline KangPeter N Schlegel
May 4, 2021·Human Fertility : Journal of the British Fertility Society·Fatemeh AsgariZahra Zandieh
May 14, 2021·Lab on a Chip·Mohammad SimchiDavid Sinton
Jul 3, 2021·Journal of Clinical Medicine·Kaan Aydos, Oya Sena Aydos
Jul 28, 2021·Zygote : the Biology of Gametes and Early Embryos·A PujolR Vassena
Nov 7, 2021·Nature Reviews. Urology·Erica T Y LeungPhilip C N Chiu

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