Microfluidic study of the chemotactic response of Escherichia coli to amino acids, signaling molecules and secondary metabolites

Biomicrofluidics
Krisztina NagyPéter Galajda

Abstract

Quorum sensing and chemotaxis both affect bacterial behavior on the population level. Chemotaxis shapes the spatial distribution of cells, while quorum sensing realizes a cell-density dependent gene regulation. An interesting question is if these mechanisms interact on some level: Does quorum sensing, a density dependent process, affect cell density itself via chemotaxis? Since quorum sensing often spans across species, such a feedback mechanism may also exist between multiple species. We constructed a microfluidic platform to study these questions. A flow-free, stable linear chemical gradient is formed in our device within a few minutes that makes it suitable for sensitive testing of chemoeffectors: we showed that the amino acid lysine is a weak chemoattractant for Escherichia coli, while arginine is neutral. We studied the effect of quorum sensing signal molecules of Pseudomonas aeruginosa on E. coli chemotaxis. Our results show that N-(3-oxododecanoyl)-homoserine lactone (oxo-C12-HSL) and N-(butryl)-homoserine lactone (C4-HSL) are attractants. Furthermore, we tested the chemoeffector potential of pyocyanin and pyoverdine, secondary metabolites under a quorum sensing control. Pyocyanin is proved to be a weak attractant while ...Continue Reading

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Citations

Apr 23, 2017·Brain, Behavior, and Immunity·Ádám Nyúl-TóthIstván A Krizbai
Apr 7, 2020·Lab on a Chip·Nicolas Garcia-SeydaOlivier Theodoly
Nov 15, 2018·Micromachines·Alireza Karbalaei, Hyoung Jin Cho
Apr 9, 2020·Micromachines·Utku M SonmezLance A Davidson
Mar 17, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Khanh L LyXiaolong Luo

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