Microfluidic system for generation of sinusoidal glucose waveforms for entrainment of islets of Langerhans.

Analytical Chemistry
Xinyu ZhangMichael G Roper

Abstract

A microfluidic system was developed to produce sinusoidal waveforms of glucose to entrain oscillations of intracellular [Ca(2+)] in islets of Langerhans. The work described is an improvement to a previously reported device where two pneumatic pumps delivered pulses of glucose and buffer to a mixing channel. The mixing channel acted as a low pass filter to attenuate these pulses to produce the desired final concentration. Improvements to the current device included increasing the average pumping frequency from 0.83 to 3.33 Hz by modifying the on-chip valves to minimize adhesion between the PDMS and glass within the valve. The cutoff frequency of the device was increased from 0.026 to 0.061 Hz for sinusoidal fluorescein waves by shortening the length of the mixing channel to 3.3 cm. The value of the cutoff frequency was chosen between the average pumping frequency and the low frequency (approximately 0.0056 Hz) glucose waves that were needed to entrain the islets of Langerhans. In this way, the pulses from the pumps were attenuated greatly but the low-frequency glucose waves were not. With the use of this microfluidic system, a total flow rate of 1.5 +/- 0.1 microL min(-1) was generated and used to deliver sinusoidal waves of glu...Continue Reading

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Citations

Sep 14, 2010·Analytical and Bioanalytical Chemistry·Liaoran CaoMichael G Roper
Jan 31, 2012·Analytical and Bioanalytical Chemistry·Jonathan G ShackmanRobert T Kennedy
Jul 21, 2011·American Journal of Physiology. Endocrinology and Metabolism·Xinyu ZhangMichael G Roper
Nov 19, 2013·Microfluidics and Nanofluidics·Xinyu ZhangMichael G Roper
Nov 27, 2015·Journal of Chromatography. a·Joshua J KlaenePaul Vouros
May 24, 2014·Biophysical Journal·Raghuram DhumpaMichael G Roper
Jun 17, 2016·Annual Review of Analytical Chemistry·Adrian M SchrellMichael G Roper
Jul 28, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Raghuram DhumpaMichael G Roper
Nov 13, 2017·Biomedical Microdevices·Qiyue LuanAbhinav Bhushan
Aug 19, 2011·Biomedical Microdevices·Dongyoung LeeJosé Oberholzer
Apr 8, 2017·Royal Society Open Science·Christopher H EmfingerColin G Nichols
May 2, 2017·Analytical Methods : Advancing Methods and Applications·Adrian M SchrellMichael G Roper
Jan 22, 2021·Diabetes·Aaron L GliebermanKevin Kit Parker
Dec 24, 2011·Analytical Chemistry·Kennon S Deal, Christopher J Easley

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