Microfluidic system for generation of sinusoidal glucose waveforms for entrainment of islets of Langerhans.

Analytical Chemistry
Xinyu ZhangMichael G Roper


A microfluidic system was developed to produce sinusoidal waveforms of glucose to entrain oscillations of intracellular [Ca(2+)] in islets of Langerhans. The work described is an improvement to a previously reported device where two pneumatic pumps delivered pulses of glucose and buffer to a mixing channel. The mixing channel acted as a low pass filter to attenuate these pulses to produce the desired final concentration. Improvements to the current device included increasing the average pumping frequency from 0.83 to 3.33 Hz by modifying the on-chip valves to minimize adhesion between the PDMS and glass within the valve. The cutoff frequency of the device was increased from 0.026 to 0.061 Hz for sinusoidal fluorescein waves by shortening the length of the mixing channel to 3.3 cm. The value of the cutoff frequency was chosen between the average pumping frequency and the low frequency (approximately 0.0056 Hz) glucose waves that were needed to entrain the islets of Langerhans. In this way, the pulses from the pumps were attenuated greatly but the low-frequency glucose waves were not. With the use of this microfluidic system, a total flow rate of 1.5 +/- 0.1 microL min(-1) was generated and used to deliver sinusoidal waves of glu...Continue Reading


Nov 8, 1979·The New England Journal of Medicine·D A LangR C Turner
Mar 1, 1991·The Journal of Clinical Endocrinology and Metabolism·G PaolissoP J Lefèbvre
Jan 1, 1990·Diabetes·H F Chou, E Ipp
May 12, 1988·The New England Journal of Medicine·S O'RahillyD R Matthews
May 12, 1988·The New England Journal of Medicine·K S PolonskyE Van Cauter
Jun 1, 1987·Diabetes·D S Weigle
Jun 27, 2000·Diabetes/metabolism Research and Reviews·P Bergsten
Jan 30, 2002·Diabetes·Patrick GilonJean-Claude Henquin
May 6, 2004·American Journal of Physiology. Endocrinology and Metabolism·Milos Zarkovic, Jean-Claude Henquin
Apr 19, 2005·Biophysical Journal·Morten Gram PedersenArthur Sherman
Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jessica OlofssonOwe Orwar
Nov 10, 2005·American Journal of Physiology. Endocrinology and Metabolism·R A RitzelP C Butler
Aug 2, 2007·American Journal of Physiology. Endocrinology and Metabolism·Richard BertramLeslie S Satin
Nov 16, 2007·Analytical Chemistry·Helen BridleOwe Orwar
Apr 25, 2008·Lab on a Chip·Y XieC H Mastrangelo
May 24, 2008·Lab on a Chip·F Azizi, C H Mastrangelo
May 24, 2008·Lab on a Chip·William H GroverScott R Manalis
Aug 1, 2008·Nature·Matthew R BennettJeff Hasty
Mar 17, 2009·PloS One·Brandon KuczenskiPhilip R Leduc
May 30, 2009·Analytical Chemistry·Alar AinlaAldo Jesorka
Aug 12, 2009·Nature Reviews. Genetics·Matthew R Bennett, Jeff Hasty
Apr 2, 2010·Lab on a Chip·Bernhard K WunderlichAndreas R Bausch

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